Influence of exercise amount and intensity on long-term weight loss maintenance and skeletal muscle mitochondrial ROS production in humans

• Published in: Applied physiology, nutrition, and metabolism Vol. 44; no. 9; pp. 958 - 964
• Main Authors: Larsen, Steen, Dandanell, Sune, Kristensen, Kasper Birch, Jørgensen, Sofie Drevsholt, Dela, Flemming, Helge, Jørn W
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.09.2019; Canadian Science Publishing NRC Research Press
• Subjects: Adult; Body mass index; contenu mitochondrial; Dextrose; Exercise; Exercise - physiology; Female; fonction mitochondriale; Glucose; glucose tolerance; Homeostasis; Humans; Influence; Lifestyles; Liraglutide; Male; Mitochondria, Muscle - metabolism; mitochondrial content; mitochondrial function; muscle squelettique; Muscle, Skeletal - physiology; Muscles; Musculoskeletal system; perte de poids; production d’espèces réactives à l’oxygène; Proteins; Reactive Oxygen Species - metabolism; reactive oxygen species production; skeletal muscle; tolérance au glucose; weight loss; Weight Loss - physiology; Weight loss maintenance; Denmark; reactive oxygen species production; mitochondrial function; muscle squelettique; contenu mitochondrial; mitochondrial content; production d’espèces réactives à l’oxygène; fonction mitochondriale; weight loss; glucose tolerance; skeletal muscle; tolérance au glucose; perte de poids
• ISSN: 1715-5312; 1715-5320
• DOI: 10.1139/apnm-2018-0577

Sustaining a weight loss after a lifestyle intervention is challenging. The objective of the present study was to investigate if mitochondrial function is associated with the ability to maintain a weight loss. Sixty-eight former participants in an 11–12-week lifestyle intervention were recruited into 2 groups; weight loss maintenance (WLM; body mass index (BMI): 32 ± 1 kg/m 2 ) and weight regain (WR; BMI: 43 ± 2 kg/m 2 ) based on weight loss measured at a follow-up visit (WLM: 4.8 ± 0.4; WR: 7.6 ± 0.8 years after lifestyle intervention). Maximal oxygen consumption rate, physical activity level, and blood and muscle samples were obtained at the follow-up experiment. Mitochondrial respiratory capacity and reactive oxygen species (ROS) production were measured. Fasting blood samples were used to calculate glucose homeostasis index. WR had impaired glucose homeostasis and decreased maximal oxygen uptake and physical activity level compared with WLM. The decreased physical activity in WR was due to a lower activity level at vigorous and moderate intensities. Mitochondrial respiratory capacity and citrate synthase (CS) activity was higher in WLM, but intrinsic mitochondrial respiratory capacity (mitochondrial respiratory capacity corrected for mitochondrial content (CS activity)) was similar. ROS production was higher in WR compared with WLM, which was accompanied by a decreased content of antioxidant proteins in WR. Intrinsic mitochondrial respiratory capacity in skeletal muscle is not associated with the ability to maintain a long-term weight loss. WLM had a higher maximal oxygen uptake, physical activity level, mitochondrial respiratory capacity and CS activity compared with WR. The reduced glucose tolerance was concurrent with increased ROS production per mitochondria in WR, and could also be associated with the lower physical activity level in this group.