Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants

• Published in: AoB plants Vol. 9; no. 3; p. plx015
• Main Authors: Erickson, David L., Reed, Elizabeth, Ramachandran, Padmini, Bourg, Norman A., McShea, William J., Ottesen, Andrea
• Format: Journal Article
• Language: English
• Published: Oxford University Press 01.05.2017
• Subjects: Invited Review; Deer diet; forest; exotic plants; metagenomics; white-tailed deer; oaks; fecal DNA; North America
• ISSN: 2041-2851; 2041-2851
• DOI: 10.1093/aobpla/plx015

US forests are plagued by chronic deer feeding on native plants and the invasion of exotic plants. One possible synergy of these issues is that white-tailed deer preferentially consume native plants and ignore exotic plants. This issue is difficult to test due to the inability to closely measure deer feeding. We used a DNA barcoding protocol to examine the plant DNA found in deer feces at a Virginia site. We identified most plant species and found exotics, as well as native plants, in the feces examined. Deer apparently avoid some exotic plants but are not the sole reason for the abundance of most exotics. Abstract Next Generation Sequencing and the application of metagenomic analyses can be used to answer questions about animal diet choice and study the consequences of selective foraging by herbivores. The quantification of herbivore diet choice with respect to native versus exotic plant species is particularly relevant given concerns of invasive species establishment and their effects on ecosystems. While increased abundance of white-tailed deer (Odocoileus virginianus) appears to correlate with increased incidence of invasive plant species, data supporting a causal link is scarce. We used a metabarcoding approach (PCR amplicons of the plant rbcL gene) to survey the diet of white-tailed deer (fecal samples), from a forested site in Warren County, Virginia with a comprehensive plant species inventory and corresponding reference collection of plant barcode and chloroplast sequences. We sampled fecal pellet piles and extracted DNA from 12 individual deer in October 2014. These samples were compared to a reference DNA library of plant species collected within the study area. For 72 % of the amplicons, we were able to assign taxonomy at the species level, which provides for the first time—sufficient taxonomic resolution to quantify the relative frequency at which native and exotic plant species are being consumed by white-tailed deer. For each of the 12 individual deer we collected three subsamples from the same fecal sample, resulting in sequencing 36 total samples. Using Qiime, we quantified the plant DNA found in all 36 samples, and found that variance within samples was less than variance between samples (F = 1.73, P = 0.004), indicating additional subsamples may not be necessary. Species level diversity ranged from 60 to 93 OTUs per individual and nearly 70 % of all plant sequences recovered were from native plant species. The number of species detected did reduce significantly (range 4–12) when we excluded species whose OTU composed <1 % of each sample’s total. When compared to the abundance of native and non-natives plants inventoried in the local community, our results support the observation that white-tailed deer have strong foraging preferences, but these preferences were not consistent for species in either class. Deer forage behaviour may favour some exotic species, but not all.