Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction

• Published in: Analytical and bioanalytical chemistry Vol. 390; no. 8; pp. 2141 - 2150
• Main Authors: Song, Suquan, Wu, Aibo, Shi, Xizhi, Li, Rongxiu, Lin, Zhixin, Zhang, Dabing
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2008; Springer-Verlag
• Subjects: Adsorption; Analytical Chemistry; Animals; Binding Sites; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography, High Pressure Liquid - methods; erythromycin; Erythromycin - analysis; Erythromycin - pharmacokinetics; Food Science; Laboratory Medicine; Methacrylates - chemistry; Molecular Imprinting - methods; Molecular Structure; Molecularly imprinted polymers; Monitoring/Environmental Analysis; Muscle, Smooth - chemistry; Original Paper; Particle Size; Pig muscle; Polymers - chemical synthesis; Polymers - chemistry; Reproducibility of Results; Sensitivity and Specificity; solid phase extraction; Solid Phase Extraction - methods; Swine; Tissue sample; Water Pollutants, Chemical - analysis; Water Supply - analysis; Molecularly imprinted polymers; Erythromycin; Tissue sample; Solid-phase extraction; Pig muscle
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-008-1985-0

Six molecularly imprinted polymers (MIPs) of erythromycin (ERY) were prepared by noncovalent bulk polymerization using methacrylic acid (MAA) as the functional monomer. On the basis of binding analysis, the MIPs with 1:2 optimum ratio of template to MAA were selected for subsequent scanning electron microscopy and Brunauer-Emmett-Teller analyses, which indicated that the MIPs had more convergent porous structures than the nonimprinted polymers. The equilibrium binding experiments showed that the binding sites of MIPs were heterogeneous, with two dissociation constants of 0.005 and 0.63 mg mL⁻¹, respectively. Furthermore, the performance of the MIPs as solid-phase extraction (SPE) sorbents was evaluated, and the selectivity analysis showed that the MIPs could recognize ERY with moderate cross-reactivity for other macrolides. The overall investigation of molecularly imprinted SPE for cleanup and enrichment of the ERY in pig muscle and tap water confirmed the feasibility of utilizing the MIPs obtained as specific SPE sorbents for ERY extraction in real samples. [graphic removed]