Molecular Cloning and DNA Sequence of Rat Amelogenin and a Comparative Analysis of Mammalian Amelogenin Protein Sequence Divergence

The developing rat incisor is a common model used in the study of enamel development. It has been impossible to study correlation between rat enamel structure and the sequence of the major developing enamel protein in this species as to date a DNA sequence for rat amelogenin has not been reported. T...

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Published inBiochemical and biophysical research communications Vol. 198; no. 2; pp. 755 - 763
Main Authors Bonass, W.A., Robinson, P.A., Kirkham, J., Shore, R.C., Robinson, C.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 28.01.1994
Elsevier
Subjects
Amelogenin
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Biological Evolution
Cloning, Molecular
Dental Enamel Proteins - genetics
DNA, Complementary - genetics
Fundamental and applied biological sciences. Psychology
Genes. Genome
Genetic Variation
Incisor - chemistry
Male
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Rats
Rats, Wistar
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tooth Germ - chemistry
Rat
Nucleotide sequence
Interspecific comparison
Rodentia
Sequence alignment
Proteins
Tooth enamel
Molecular evolution
Vertebrata
Mammalia
Complementary DNA
Gene
Gene duplication
Molecular cloning
Amelogenin
Aminoacid sequence
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Summary:The developing rat incisor is a common model used in the study of enamel development. It has been impossible to study correlation between rat enamel structure and the sequence of the major developing enamel protein in this species as to date a DNA sequence for rat amelogenin has not been reported. This study presents the first cloning of a full-length cDNA copy of rat amelogenin and its deduced primary sequence. Detailed analysis of this sequence provides evidence that the gene has evolved by internal sequence duplication. Comparison of the rat amelogenin primary sequence with those published for other species provides evidence that this protein, while exhibiting extreme levels of sequence conservation, has been subject to significant structural changes that may be related to alterations in enamel structure in different mammalian groups.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1994.1109