Cell cycle dependence of mitotic delay in X-irradiated normal and ataxia-telangiectasia fibroblasts

The delay in progression of X-irradiated cells through the cell cycle, which is more pronounced in normal (N) than in A-T fibroblasts, is greatest for cells in G2 at the time of irradiation. The greater effect of radiation on the initiation of DNA synthesis in N than in A-T cells is reflected in the...

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Published inInternational journal of radiation biology and related studies in physics, chemistry and medicine Vol. 42; no. 6; p. 679
Main Authors Scott, D, Zampetti-Bosseler, F
Format Journal Article
LanguageEnglish
Published England 01.01.1982
Subjects
Ataxia Telangiectasia - pathology
Cell Cycle
Cell Line
Fibroblasts - radiation effects
Humans
Mitosis - radiation effects
Mitotic Index - radiation effects
Skin - cytology
Time Factors
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Summary:The delay in progression of X-irradiated cells through the cell cycle, which is more pronounced in normal (N) than in A-T fibroblasts, is greatest for cells in G2 at the time of irradiation. The greater effect of radiation on the initiation of DNA synthesis in N than in A-T cells is reflected in the shape of the percent labelled mitosis curves after 3H-thymidine treatment. The duration of the S phase in unirradiated A-T cells is greater than in N cells. Any explanation of the underlying defect in A-T must account not only for the reduced radiosensitivity of DNA synthesis but for the lesser delay in G2. Our data support the hypothesis that DNA is the principal target for radiation-induced G2 delay.
ISSN:0020-7616
DOI:10.1080/09553008214551661