Single-cell RNA sequencing of mouse brain and lung vascular and vessel-associated cell types

• Published in: Scientific data Vol. 5; no. 1; p. 180160
• Main Authors: He, Liqun, Vanlandewijck, Michael, Mäe, Maarja Andaloussi, Andrae, Johanna, Ando, Koji, Del Gaudio, Francesca, Nahar, Khayrun, Lebouvier, Thibaud, Laviña, Bàrbara, Gouveia, Leonor, Sun, Ying, Raschperger, Elisabeth, Segerstolpe, Åsa, Liu, Jianping, Gustafsson, Sonja, Räsänen, Markus, Zarb, Yvette, Mochizuki, Naoki, Keller, Annika, Lendahl, Urban, Betsholtz, Christer
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.08.2018; Nature Publishing Group
• Subjects: 631/1647/2017; 631/1647/334/1874/345; 631/1647/514/1949; 631/378/340; Animals; Blood vessels; Blood Vessels - cytology; Blood Vessels - physiology; Brain - blood supply; Data Descriptor; Databases, Factual; Datasets; Endothelial Cells - physiology; Gene expression; Humanities and Social Sciences; Lung - blood supply; Lungs; Mice; multidisciplinary; Myocytes, Smooth Muscle - physiology; Pericytes - physiology; Ribonucleic acid; RNA; Rodents; Science; Sequence Analysis, RNA; Single-Cell Analysis; Structure-function relationships; Transcriptome; Vascular diseases
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/sdata.2018.160

Vascular diseases are major causes of death, yet our understanding of the cellular constituents of blood vessels, including how differences in their gene expression profiles create diversity in vascular structure and function, is limited. In this paper, we describe a single-cell RNA sequencing (scRNA-seq) dataset that defines vascular and vessel-associated cell types and subtypes in mouse brain and lung. The dataset contains 3,436 single cell transcriptomes from mouse brain, which formed 15 distinct clusters corresponding to cell (sub)types, and another 1,504 single cell transcriptomes from mouse lung, which formed 17 cell clusters. In order to allow user-friendly access to our data, we constructed a searchable database ( http://betsholtzlab.org/VascularSingleCells/database.html ). Our dataset constitutes a comprehensive molecular atlas of vascular and vessel-associated cell types in the mouse brain and lung, and as such provides a strong foundation for future studies of vascular development and diseases. Design Type(s) organism part comparison design • parallel group design • transcription profiling design • database creation objective Measurement Type(s) transcription profiling assay Technology Type(s) single cell sequencing Factor Type(s) experimental condition • genotype Sample Characteristic(s) Mus musculus • brain vasculature • lung vasculature Machine-accessible metadata file describing the reported data (ISA-Tab format)