Establishing optimal selenium status: results of a randomized, double-blind, placebo-controlled trial

• Published in: The American journal of clinical nutrition Vol. 91; no. 4; pp. 923 - 931
• Main Authors: Hurst, Rachel, Armah, Charlotte N, Dainty, Jack R, Hart, Dave J, Teucher, Birgit, Goldson, Andrew J, Broadley, Martin R, Motley, Amy K, Fairweather-Tait, Susan J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Clinical Nutrition 01.04.2010; American Society for Nutrition; American Society for Clinical Nutrition, Inc
• Subjects: administration & dosage; adults; Biological and medical sciences; biomarkers; Biomarkers - blood; blood; blood chemistry; blood platelets; Blood Platelets - drug effects; Diet; dietary mineral supplements; Dietary minerals; dietary nutrient sources; dietary recommendations; Dietary Supplements; Double-Blind Method; drug effects; enriched foods; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; glutathione peroxidase; Glutathione Peroxidase - blood; Great Britain; Humans; Male; Medical research; men; Middle Aged; nutrient requirements; Nutrition; nutritional intervention; Nutritional Requirements; Nutritional Status; Nutritional status, dietary intake, and body composition; Onions; Original Research Communications; Plasma; Proteins; reference standards; Selenium; Selenium - administration & dosage; Selenium - blood; Selenoprotein P; Selenoprotein P - blood; Trace Elements; Trace Elements - administration & dosage; Trace Elements - blood; United Kingdom; Vertebrates: anatomy and physiology, studies on body, several organs or systems; women; Yeasts; United Kingdom; United Kingdom > UK; Great Britain; Human; Double blind study; Selenium
• ISSN: 0002-9165; 1938-3207; 1938-3207
• DOI: 10.3945/ajcn.2009.28169

BACKGROUND: Dietary recommendations for selenium differ between countries, mainly because of uncertainties over the definition of optimal selenium status. OBJECTIVE: The objective was to examine the dose-response relations for different forms of selenium. DESIGN: A randomized, double-blind, placebo-controlled dietary intervention was carried out in 119 healthy men and women aged 50-64 y living in the United Kingdom. Daily placebo or selenium-enriched yeast tablets containing 50, 100, or 200 μg Se ([almost equal to]60% selenomethionine), selenium-enriched onion meals ([almost equal to]66% γ-glutamyl-methylselenocysteine, providing the equivalent of 50 μg Se/d), or unenriched onion meals were consumed for 12 wk. Changes in platelet glutathione peroxidase activity and in plasma selenium and selenoprotein P concentrations were measured. RESULTS: The mean baseline plasma selenium concentration for all subjects was 95.7 ± 11.5 ng/mL, which increased significantly by 10 wk to steady state concentrations of 118.3 ± 13.1, 152.0 ± 24.3, and 177.4 ± 26.3 ng/mL in those who consumed 50, 100, or 200 μg Se-yeast/d, respectively. Platelet glutathione peroxidase activity did not change significantly in response to either dose or form of selenium. Selenoprotein P increased significantly in all selenium intervention groups from an overall baseline mean of 4.99 ± 0.80 μg/mL to 6.17 ± 0.85, 6.73 ± 1.01, 6.59 ± 0.64, and 5.72 ± 0.75 μg/mL in those who consumed 50, 100, or 200 μg Se-yeast/d and 50 μg Se-enriched onions/d, respectively. CONCLUSIONS: Plasma selenoprotein P is a useful biomarker of status in populations with relatively low selenium intakes because it responds to different dietary forms of selenium. To optimize the plasma selenoprotein P concentration in this study, 50 μg Se/d was required in addition to the habitual intake of [almost equal to]55 μg/d. In the context of established relations between plasma selenium and risk of cancer and mortality, and recognizing the important functions of selenoprotein P, these results provide important evidence for deriving estimated average requirements for selenium in adults. This trial was registered at clinicaltrials.gov as NCT00279812.