Single-Cell Quantitative HER2 Measurement Identifies Heterogeneity and Distinct Subgroups within Traditionally Defined HER2-Positive Patients

• Published in: The American journal of pathology Vol. 183; no. 5; pp. 1446 - 1460
• Main Authors: Onsum, Matthew D, Geretti, Elena, Paragas, Violette, Kudla, Arthur J, Moulis, Sharon P, Luus, Lia, Wickham, Thomas J, McDonagh, Charlotte F, MacBeath, Gavin, Hendriks, Bart S
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2013
• Subjects: Animals; Breast Neoplasms - classification; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line, Tumor; Cluster Analysis; Female; Fluorescent Antibody Technique; Genetic Heterogeneity; Humans; Mice; Mice, Nude; Neoplasms - classification; Neoplasms - metabolism; Neoplasms - pathology; Pathology; Receptor, ErbB-2 - metabolism; Reference Standards; Reproducibility of Results; Single-Cell Analysis - methods; Stomach Neoplasms - classification; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Tissue Array Analysis; Urinary Bladder Neoplasms - classification; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology
• ISSN: 0002-9440; 1525-2191
• DOI: 10.1016/j.ajpath.2013.07.015

Human epidermal growth factor receptor 2 (HER2) is an important biomarker for breast and gastric cancer prognosis and patient treatment decisions. HER2 positivity, as defined by IHC or fluorescent in situ hybridization testing, remains an imprecise predictor of patient response to HER2-targeted therapies. Challenges to correct HER2 assessment and patient stratification include intratumoral heterogeneity, lack of quantitative and/or objective assays, and differences between measuring HER2 amplification at the protein versus gene level. We developed a novel immunofluorescence method for quantitation of HER2 protein expression at the single-cell level on FFPE patient samples. Our assay uses automated image analysis to identify and classify tumor versus non-tumor cells, as well as quantitate the HER2 staining for each tumor cell. The HER2 staining level is converted to HER2 protein expression using a standard cell pellet array stained in parallel with the tissue sample. This approach allows assessment of HER2 expression and heterogeneity within a tissue section at the single-cell level. By using this assay, we identified distinct subgroups of HER2 heterogeneity within traditional definitions of HER2 positivity in both breast and gastric cancers. Quantitative assessment of intratumoral HER2 heterogeneity may offer an opportunity to improve the identification of patients likely to respond to HER2-targeted therapies. The broad applicability of the assay was demonstrated by measuring HER2 expression profiles on multiple tumor types, and on normal and diseased heart tissues.