Characterization of two putative fibronectin-binding proteins of Clostridium perfringens
Clostridium perfringens is a Gram-positive anaerobic pathogen that causes gas gangrene and food poisoning in humans and animals. Genomic analysis of C. perfringens strain 13 revealed that this bacterium contains two genes (CPE0737 and CPE1847) that encode putative fibronectin (Fn)-binding proteins (...
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Published in | Anaerobe Vol. 15; no. 4; pp. 155 - 159 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.08.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Clostridium perfringens is a Gram-positive anaerobic pathogen that causes gas gangrene and food poisoning in humans and animals. Genomic analysis of
C. perfringens strain 13 revealed that this bacterium contains two genes (CPE0737 and CPE1847) that encode putative fibronectin (Fn)-binding proteins (Fbps). These genes, named
fbpA and
fbpB, were found to be constitutively expressed in all three strains (13, NCTC8237, CPN50) of
C. perfringens, isolated from gas gangrene of human, that were tested. Both
fbpA and
fbpB were cloned and His-tagged, recombinant FbpA (rFbpA) and recombinant FbpB (rFbpB) were purified by Ni
2+-Sepharose column chromatography from transformed
Escherichia coli. These recombinant Fbps were shown to bind to Fn, purified from human serum, in a ligand blotting assay. Additionally, Fn bound to these rFbps in a dose-dependent manner in an enzyme-linked immunosorbent assay. Furthermore, it was found that pre-incubation of Fn with either rFbpA or rFbpB inhibited the binding of Fn to
C. perfringens cells. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1075-9964 1095-8274 |
DOI: | 10.1016/j.anaerobe.2009.03.001 |