Methods for implanting steroid-containing cannulae into the paraventricular nucleus of neonatal rats

Implantation of cannulae into brains of neonatal rats presents methodological difficulties. We discuss such issues as avoiding tissue injury, and describe successful techniques. Cannulae size, methods of preparation, insertion, and securing are evaluated. We present a modified cannula holder applica...

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Bibliographic Details
Published inJournal of pharmacological and toxicological methods Vol. 30; no. 2; p. 97
Main Authors Yi, S J, Baram, T Z
Format Journal Article
LanguageEnglish
Published United States 01.10.1993
Subjects
Animals
Animals, Newborn
Catheterization - methods
Cholesterol - administration & dosage
Cholesterol - pharmacology
Corticotropin-Releasing Hormone - genetics
Female
Gene Expression - drug effects
Mifepristone - administration & dosage
Mifepristone - pharmacology
Paraventricular Hypothalamic Nucleus
Pregnancy
Rats
Rats, Sprague-Dawley
Weight Gain - drug effects
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Summary:Implantation of cannulae into brains of neonatal rats presents methodological difficulties. We discuss such issues as avoiding tissue injury, and describe successful techniques. Cannulae size, methods of preparation, insertion, and securing are evaluated. We present a modified cannula holder applicable to the soft neonatal brain. Application of these methods to the study of glucocorticoid receptors in the neonatal rat hypothalamus is illustrated.
ISSN:1056-8719
1873-488X
DOI:10.1016/1056-8719(93)90012-4