A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus

• Published in: Nature genetics Vol. 21; no. 2; pp. 157 - 158
• Main Authors: Andersson, Leif, Jeon, Jin-Tae, Carlborg, Örjan, Törnsten, Anna, Giuffra, Elisabetta, Amarger, Valerie, Chardon, Patrick, Andersson-Eklund, Lena, Andersson, Kjell, Hansson, Ingemar, Lundström, Kerstin
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01.02.1999
• Subjects: Animal biology; Animals; Chromosome Mapping; Fathers; Female; Genetics; Genetik; Insulin-Like Growth Factor II - genetics; Life Sciences; Male; Muscle, Skeletal - metabolism; Myocardium - metabolism; Quantitative Trait, Heritable; Swine; muscle cardiaque; cartographie génétique
• ISSN: 1061-4036; 1546-1718; 1546-1718
• DOI: 10.1038/5938

We generated an intercross between the European Wild Boar and Large White domestic pigs and used this pedigree to map quantitative trait loci (QTL). We previously reported a QTL on pig chromosome 2p with a moderate effect on muscle mass. The conserved synteny between this region and human chromosome 11p suggested IGF2 as a candidate for the QTL. We isolated a porcine BAC IGF2 clone (253G10) and used it for FISH mapping, resulting in a consistent signal on the distal tip of chromosome 2p (band 2p1.7). A microsatellite is located in the IGF2 3 theta UTR in mice (GenBank U71085), humans (GenBank S62623) and horse (GenBank AF020598). Direct BAC sequencing of the IGF2 3 theta UTR revealed a microsatellite 800 bp downstream of the IGF2 stop codon identical to a previously described microsatellite, Swc9 (ref. 5). The IGF2 microsatellite was found to be highly polymorphic, with three alleles among the two Wild Boar founders and another two among the eight Large White founders, and was fully informative in the intercross, as the breed and parent of origin could be determined for each allele in F sub(2) animals. Linkage analysis showed that IGF2 maps 25 cM distal to Sw256, the end marker in our previous study (Fig. 1). The Swr2516/Sw2443 markers represent the end of the known linkage map; FISH mapping of IGF2 indicated that they are close to the telomere.