Structural and Functional Changes of Groundwater Bacterial Community During Temperature and pH Disturbances

• Published in: Microbial ecology Vol. 78; no. 2; pp. 428 - 445
• Main Authors: Song, Yuhao, Mao, Guannan, Gao, Guanghai, Bartlam, Mark, Wang, Yingying
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media 01.08.2019; Springer US; Springer Nature B.V
• Subjects: Ammonia-oxidizing bacteria; Bacteria; Biomedical and Life Sciences; Communities; Denitrification; Disturbances; Ecology; ENVIRONMENTAL MICROBIOLOGY; Filterability; Flow cytometry; Genes; Geoecology/Natural Processes; Groundwater; Life Sciences; Microbial Ecology; Microbiology; Microorganisms; Nature Conservation; Next-generation sequencing; Nitrification; Nitrogen; Nucleic acids; pH effects; Phylogeny; Proteobacteria; Relative abundance; Structure-function relationships; Temperature effects; Temperature tolerance; Water analysis; Water Quality/Water Pollution; Water sampling; Flow cytometry; Filtration; Groundwater; Microbial community structure; Nitrogen-cycling functional genes
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-019-01333-7

In this study, we report the characteristics of a microbial community in sampled groundwater and elucidate the effects of temperature and pH disturbances on bacterial structure and nitrogen-cycling functions. The predominant phyla of candidate OD1, candidate OP3, and Proteobacteria represented more than half of the total bacteria, which clearly manifested as a “low nucleic acid content (LNA) bacteria majority” type via flow cytometric fingerprint. The results showed that LNA bacteria were more tolerant to rapid changes in temperature and pH, compared to high nucleic acid content (HNA) bacteria. A continuous temperature increase test demonstrated that the LNA bacterial group was less competitive than the HNA bacterial group in terms of maintaining their cell intactness and growth potential. In contrast, the percentage of intact LNA bacteria was maintained at nearly 70% with pH decrease, despite a 50% decrease in total intact cells. Next-generation sequencing results revealed strong resistance and growth potential of phylum Proteobacteria when the temperature increased or the pH decreased in groundwater, especially for subclasses α-, ß-, and γ-Proteobacteria. In addition, relative abundance of nitrogen-related functional genes by qPCR showed no difference in nitrifiers or denitrifiers within 0.45 µm-captured and 0.45 µm-filterable bacteria due to phylogenetic diversity. One exception was the monophyletic anammox bacteria that belong to the phylum Planctomycetes, which were mostly captured on a 0.45-µm filter. Furthermore, we showed that both temperature increase and pH decrease could enhance the denitrification potential, whereas the nitrification and anammox potentials were weakened.