No Age-Related Change in Circulating Endothelial Progenitor Cells in Healthy Subjects

• Published in: International Heart Journal Vol. 47; no. 1; pp. 95 - 105
• Main Authors: Chen, Mien-Cheng, Yip, Hon-Kan, Chen, Chien-Jen, Yang, Cheng-Hsu, Wu, Chiung-Jen, Cheng, Cheng-I, Chen, Yen-Hsun, Chai, Han-Tan, Lee, Ching-Ping, Chang, Hsueh-Wen
• Format: Journal Article
• Language: English
• Published: Japan International Heart Journal Association 2006
• Subjects: Adult; Age; Age Factors; Antigens, CD34 - blood; Cytokine; E-Selectin - blood; Endothelial Cells - cytology; Endothelial progenitor cell; Female; Flow Cytometry; Humans; Male; Middle Aged; Platelet Endothelial Cell Adhesion Molecule-1 - blood; Risk Factors; Stem cell; Stem Cells - cytology
• ISSN: 1349-2365; 1349-3299
• DOI: 10.1536/ihj.47.95

Endothelial progenitor cells were isolated from peripheral blood obtained from 32 healthy volunteers without cardiovascular risk factors who ranged in age from 20 to 61 years (mean [± SD] age, 34.1 ± 9.6 years). The fractions of CD34+ endothelial progenitor cells expressing kinase insert domain receptor-1, CD62E, or CD31 were analyzed with flow cytometry. Correlation analysis demonstrated that there was no significant correlation between subject age and the fraction of circulating CD34+ mononuclear cells expressing kinase insert domain receptor-1 (P = 0.324; r = −0.180). Similarly, there was no significant correlation between subject age and the fraction of circulating CD34+ mononuclear cells expressing CD62E (P = 0.496; r = −0.125) or the fraction of circulating CD34+ mononuclear cells expressing CD31 (P = 0.245; r = −0.212). In conclusion, the experimental results showed that there was no age-related change in the basal level of circulating endothelial progenitor cells in healthy subjects without cardiovascular risk factors.