One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal

A purification technique to obtain keratinolytic proteases produced by Bacillus sp. P45 in a medium containing chicken feather meal as substrate is presented. The experiments were carried out in a dead-end ultrafiltration unit, and the influence of the membrane cutoff, pH of enzymatic extract, and o...

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Bibliographic Details
Published inInternational Journal of Chemical Engineering Vol. 2018; no. 2018; pp. 1 - 7
Main Authors Brandelli, Adriano, Ores, Joana da C., de Oliveira, Janaina M. G., Severo, Emanuelle E., Machado, Juliana R., Kalil, Susana J.
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2018
Hindawi
Hindawi Limited
Wiley
Subjects
Amino acids
Biodegradation
Biotechnology industry
Chromatography
Enzymes
Feeds
Keratin
Membrane separation
Molecular chains
Poultry
Proteins
Purification
Substrates
Ultrafiltration
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Summary:A purification technique to obtain keratinolytic proteases produced by Bacillus sp. P45 in a medium containing chicken feather meal as substrate is presented. The experiments were carried out in a dead-end ultrafiltration unit, and the influence of the membrane cutoff, pH of enzymatic extract, and operating pressure on the purification of keratinase were studied. The one-step ultrafiltration process with the membrane molecular mass cutoff of 10 kDa at pH 8.0 and operating pressure of 0.147 MPa showed an enzyme recovery of 87.8% and a 4.1-fold purification factor. It is showed that ultrafiltration could be potentially used in the purification of keratinases.
ISSN:1687-806X
1687-8078
DOI:10.1155/2018/6729490