Virgibacillus natechei sp. nov., A Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in Southwest of Algeria

• Published in: Current microbiology Vol. 66; no. 5; pp. 462 - 466
• Main Authors: Amziane, Meriam, Metiaz, Farida, Darenfed-Bouanane, Amel, Djenane, Zahia, Selama, Okba, Abderrahmani, Ahmed, Cayol, Jean-Luc, Fardeau, Marie-Laure
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.05.2013; Springer Nature B.V; Springer Verlag
• Subjects: Algeria; Bacteria; Biodiversity; Biomedical and Life Sciences; Biotechnology; Clostridiales; DNA; fatty acids; Fatty Acids - chemistry; glucose; Gram-positive bacteria; Lakes; Lakes - microbiology; Life Sciences; Lipids; mannitol; melibiose; Microbiology; Molecular Sequence Data; nucleotide sequences; phospholipids; Phylogenetics; phylogeny; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Salinity; Salt lakes; Sediments; Sodium chloride; Systematics, Phylogenetics and taxonomy; Virgibacillus; Virgibacillus - chemistry; Virgibacillus - classification; Virgibacillus - genetics; Virgibacillus - isolation & purification; Algeria; Respiratory Quinone; Xylitol; Acetoin; Saline Lake; Halophilic Bacterium
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-012-0300-7

A novel, Gram-positive, moderately halophilic bacterium, oxidase- and catalase-positive designated FarDᵀ was isolated from sediments of a saline lake located in Taghit, 93 km from Bechar, southwest of Algeria. Cells were rod-shaped, endospore forming, and motile. Growth occurred at 15–40 °C (optimum, 35 °C), pH 6.0–12.0 (optimum, 7.0) and in the presence of 1–20 % NaCl (optimum, 10 %). Strain FarDᵀ used glucose, mannitol, melibiose, D-mannose, and 5 ketogluconate. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and three phospholipids; MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C₁₅:₀, anteiso C₁₇:₀, C₂₀:₀, and anteiso C₁₉:₀. The DNA G+C content was 42.1 mol%. Phylogenetic analysis of the small-subunit ribosomal RNA gene sequence indicated that strain FarDᵀ had as its closest relative Virgibacillus salinus (similarity of 96.3 %). Based on phenotypic, phylogenetic, and taxonomic characteristics, strain FarDᵀ is proposed as a novel species of the genus Virgibacillus within the order Clostridiales, for which the name V. natechei is proposed. The type strain is FarDᵀ (=DSM 25609ᵀ = CCUG 62224ᵀ).