The effect of antibiotics on the in vitro growth response of chrysanthemum and tobacco stem transverse thin cell layers (tTCLs)

• Published in: Scientia Horticulturae Vol. 97; no. 3; pp. 397 - 410
• Main Authors: Teixeira da Silva, Jaime A, Nhut, Duong T, Tanaka, Michio, Fukai, Seiichi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 17.02.2003; Elsevier BV; Elsevier
• Subjects: Agrobacterium; Agronomy. Soil science and plant productions; antibiotics; Biological and medical sciences; callus formation; Chrysanthemum morifolium; explants; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; in vitro regeneration; micropropagation; Nicotiana tabacum; Phytotoxicity; propagation materials; Shoot regeneration capacity; shoots; stems; Thin cell layer (TCL); Threshold survival level (TSL); tissue culture; tobacco; Vegetative propagation. Micropropagation; Vegetative propagation. Sowing and planting. Harvesting; Threshold survival level (TSL); Agrobacterium; Phytotoxicity; Thin cell layer (TCL); Shoot regeneration capacity; Tissue culture; Explant; Stimulant plant; Micropropagation; Compositae; Nicotiana tabacum; Survival; Antimicrobial agent; Dendranthema grandiflora; Regeneration; Antibiotic; Dicotyledones; Angiospermae; Flower crop; Spermatophyta; Solanaceae; Biological contamination
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/S0304-4238(02)00219-4

Plant tissue culture systems are always under threat of microbial contamination from the air, the tissue culturer, and from the tissue cultured plant itself, and antibiotics can be utilized to eliminate unwanted contaminants from tissue culture. In addition, the genetic transformation of plants requires selection and regeneration of transformed tissues through the use of an antibiotic-degrading gene. This study utilizes a wide range of antibiotic agents to examine their effect on chrysanthemum and tobacco thin cell layer (TCL) tissue culture systems, with an increasing gradient of phytotoxicity being observed: bialaphos ®>chloramphenicol>rifampicin>streptomycin>minomycin>ampicillin>penicillin G=penicillin V, demonstrating that the limiting factor to the use of these agents is in fact the plant. Following the establishment of threshold survival levels, a decrease in explant survival and reduced biomass, malformation of roots and inhibition of shoot formation in chrysanthemum was observed, and further, in the case of tobacco endoreduplication.