Linoleic acid-derived 13-hydroxyoctadecadienoic acid is absorbed and incorporated into rat tissues

• Published in: Biochimica et biophysica acta. Molecular and cell biology of lipids Vol. 1866; no. 3; p. 158870
• Main Authors: Zhang, Zhichao, Emami, Shiva, Hennebelle, Marie, Morgan, Rhianna K., Lerno, Larry A., Slupsky, Carolyn M., Lein, Pamela J., Taha, Ameer Y.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2021
• Subjects: Adipose tissue; Adipose Tissue - metabolism; Animals; Brain; Brain - metabolism; Esterification; Heart; Kinetics; Linoleic Acid - metabolism; Linoleic Acids - administration & dosage; Linoleic Acids - blood; Linoleic Acids - metabolism; Liver; Liver - metabolism; Male; Myocardium - metabolism; Oxidized linoleic acid metabolites (OXLAMs); Rats; Rats, Inbred F344; Heart; Brain; Oxidized linoleic acid metabolites (OXLAMs); Adipose tissue; Liver; 13-HODE; SPE; COX; IV; ES; OXLAMs; PGE; ARA; UE; LOX; CYP450; EPA; PG; sEH; UPLC-MS/MS; Kinetics; LNA
• ISSN: 1388-1981; 1879-2618
• DOI: 10.1016/j.bbalip.2020.158870

Linoleic acid (LNA)-derived 13-hydroxyoctadecadienoic acid (13-HODE) is a bioactive lipid mediator that regulates multiple signaling processes in vivo. 13-HODE is also produced when LNA is oxidized during food processing. However, the absorption and incorporation kinetics of dietary 13-HODE into tissues is not known. The present study measured unesterified d4-13-HODE plasma bioavailability and incorporation into rat liver, adipose, heart and brain following gavage or intravenous (IV) injection (n = 3 per group). Mass spectrometry analysis revealed that d4-13-HODE was absorbed within 20 min of gavage, and continued to incorporate into plasma esterified lipid fractions throughout the 90 min monitoring period (incorporation half-life of 71 min). Following IV injection, unesterified d4-13-HODE was rapidly eliminated from plasma with a half-life of 1 min. Analysis of tracer incorporation kinetics into rat tissues following IV injection or gavage revealed that the esterified tracer preferentially incorporated into liver, adipose and heart compared to unesterified d4-13-HODE. No tracer was detected in the brain. This study demonstrates that dietary 13-HODE is absorbed, and incorporated into peripheral tissues from esterified plasma lipid pools. Understanding the chronic effects of dietary 13-HODE exposure on peripheral tissue physiology and metabolism merits future investigation. •d4-13-hydroxyoctadecadienoic acid (13-HODE) incorporated into plasma following IV and gavage.•d4-13-HODE incorporated into liver, adipose and heart following IV and gavage.•Esterified d4-13-HODE preferentially incorporated into tissues, versus free d4-13-HODE.•Labeled 13-HODE was not detected in the brain.