Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 7
• Main Authors: Gomes, Maria A., Melo, Maria Norma, Gil, Frederico F., Fernandes, Helen C., Alvarenga, Ângela C., Freitas, Michelle A. R., Pesquero, Jorge L.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2014; Hindawi Limited
• Subjects: Animals; Biomedical research; CHO Cells; Cloning; Cricetinae; Cricetulus; Cytopathogenic Effect, Viral - genetics; Drug resistance; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Entamoeba histolytica; Entamoeba histolytica - genetics; Entamoeba histolytica - pathogenicity; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Genomes; Humans; Hybridization; Inflammatory bowel disease; Kinases; Liver; Liver - parasitology; Male; Parasites; Proteins; Rodents; Signal transduction; Subtractive Hybridization Techniques - methods; Trophozoites - physiology; Virulence - genetics
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/285607

Entamoeba histolytica is a parasite which presents capacity to degrade tissues and therefore has a pathogenic behavior. As this behavior is not shown by all strains, there have been several studies investigating molecular basis of the cytotoxicity process. Using the suppression subtractive hybridization (SSH) technique, differential gene expressions of two E. histolytica strains, one virulent (EGG) and one nonvirulent (452), have been analyzed with the purpose of isolating genes which may be involved with amoebic virulence. Nine cDNA fragments presenting high homology with E. histolytica previously sequenced genes were subtracted. Of these, four genes were confirmed by RT-PCR. Two coding for hypothetical proteins, one for a cysteine-rich protein, expressed only in the virulent strain, EGG and another one, coding for grainin 2 protein, exclusive from 452 strain. This study provided new insight into the proteins differences in the virulent and nonvirulent E. histolytica strains. We believe that further studies with these proteins may prove association of them with tissue damage, providing new perceptions to improve treatment or diagnosis of the invasive disease.