Avoidance of endotoxin-induced inflammation during studies of albumin clearance from caprine joints
Intra-articular injection of radiolabelled, commercial goat serum albumin (GSA) produces acute arthritis in caprine joints. This inflammation distorts clearance values and vitiates studies of normal lymphatic function. Endotoxin, routinely found in commercial albumin preparations, appears to cause t...
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Published in | Experimental physiology Vol. 77; no. 6; pp. 839 - 848 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge
The Physiological Society
01.11.1992
Cambridge University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Intra-articular injection of radiolabelled, commercial goat serum albumin (GSA) produces acute arthritis in caprine joints.
This inflammation distorts clearance values and vitiates studies of normal lymphatic function. Endotoxin, routinely found
in commercial albumin preparations, appears to cause this local reaction. We describe a simple method for the preparation
of low-endotoxin, radioiodinated serum albumin from aseptically collected serum. We have used this technique to prepare GSA
for use as a tracer molecule in clearance studies of synovial joint lymphatic function. The isolated protein exhibits antigenic
and chemical characteristics indistinguishable from those of commercial GSA but contains at least 1000-fold less endotoxin.
In contrast to commercial GSA preparations, this albumin does not produce local inflammation when injected into synovial joints
and is cleared from caprine joints in the normal monoexponential manner. Low levels of endotoxin seriously distort studies
of articular albumin kinetics and may induce comparable artifacts when commercial protein preparations are used to study other
physiological systems. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0958-0670 1469-445X |
DOI: | 10.1113/expphysiol.1992.sp003650 |