Generation of Footprint‐Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector

Using auto‐erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT‐PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one...

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Published inMolecular reproduction and development Vol. 87; no. 6; pp. 663 - 665
Main Authors Tsukamoto, Masaya, Kimura, Kazuto, Tanaka, Miyuu, Kuwamura, Mitsuru, Ohtaka, Manami, Nakanishi, Mahito, Sugiura, Kikuya, Hatoya, Shingo
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.06.2020
Subjects
canine induced pluripotent stem cells
Karyotypes
Leukocytes (mononuclear)
Peripheral blood mononuclear cells
Pluripotency
Sendai virus vector
SeVdp(KOSM)302L
Stem cells
peripheral blood mononuclear cells
SeVdp(KOSM)302L
Sendai virus vector
canine induced pluripotent stem cells
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Summary:Using auto‐erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT‐PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.
Bibliography:SourceType-Other Sources-1
ObjectType-Article-2
content type line 63
ObjectType-Correspondence-1
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.23349