Effects of the Neotyphodium endophyte fungus on dormancy and germination rate of Lolium multiflorum seeds

• Published in: Austral ecology Vol. 31; no. 6; pp. 767 - 775
• Main Authors: GUNDEL, PEDRO E, MASEDA, PABLO H, GHERSA, CLAUDIO M, BENECH-ARNOLD, ROBERTO L
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Melbourne, Australia : Blackwell Publishing Asia 01.09.2006; Blackwell Publishing Asia; Blackwell; Blackwell Publishing Ltd
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Biological and medical sciences; dormancy; Fundamental and applied biological sciences. Psychology; Fungi; General aspects; Germination; germination rate; Grasses; Lolium multiflorum; maternal effect; Neotyphodium; Seeds; Dormancy; Monocotyledones; Seeds; Gramineae; Germination; Lolium multiflorum; Angiospermae; Neotyphodium; Endophyte; Spermatophyta; Maternal effect; germination rate
• ISSN: 1442-9985; 1442-9993
• DOI: 10.1111/j.1442-9993.2006.01638.x

Neotyphodium frequently occurs as an endophyte in grasses. Evidence shows enhanced fitness of endophyte infected grasses relative to non-infected ones. Some studies of seed germination show endophyte enhancement of plant fitness in various environments, but inconsistent results indicate that further studies are needed. So far, experiments have failed to separate the confounded effects of population origin and seed management. For this reason, we evaluated the effects of endophyte infection on seed dormancy and germination in Lolium multiflorum using an experimental design controlling these factors. Depending on the year of seed production, endophyte infection modified seed response to light quality, affecting predominantly seed dormancy levels. Nevertheless, the endophyte did not affect base temperature or thermal time of germination. We concluded that endophytes were not a strong influence on germination behaviour. We speculate from our results that the presence of the endophyte changes germination by an indirect effect, in extending growth of the maternal plant during seed development and ripening. The direct effect of hyphae in the seed on seed behaviour was disregarded, because the difference between infected and non-infected seed varied within the year of seed production. Future experiments should focus on effects of the endophyte on the canopy of parent plants during seed production and ripening, and, hence, on subsequent dormancy and germination of the seeds.