Establishment and characterization of a subline predisposed to pulmonary metastasis from a human gestational choriocarcinoma cell line in nude mice

A subline predisposed to pulmonary metastasis was successfully derived by repeating in vivo selection of GCH-1, a human gestational choriocarcinoma cell line, in nude mice. While the parent line GCH-1, transplanted subcutaneously to nude mice, induced pulmonary metastasis in a few of the host animal...

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Bibliographic Details
Published inActa obstetricia et gynecologica Scandinavica Vol. 68; no. 5; p. 429
Main Authors Kanazawa, K, Adachi, S, Yoshiya, N, Honma, S, Takahashi, H, Takeuchi, S
Format Journal Article
LanguageEnglish
Published United States 1989
Subjects
Animals
Cell Line
Choriocarcinoma - pathology
Choriocarcinoma - secondary
Female
Humans
Lung - pathology
Lung Neoplasms - secondary
Mice
Mice, Nude
Neoplasm Transplantation
Pregnancy
Uterine Neoplasms - pathology
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Summary:A subline predisposed to pulmonary metastasis was successfully derived by repeating in vivo selection of GCH-1, a human gestational choriocarcinoma cell line, in nude mice. While the parent line GCH-1, transplanted subcutaneously to nude mice, induced pulmonary metastasis in a few of the host animals, the newly established subline GCH-1(m) successfully metastasized to the lungs in 100% of them. Compared with GCH-1, GCH-1(m) exhibited a higher degree of cell atypia, a lower capacity for cell growth and markedly higher productivity for human chorionic gonadotropin (hCG). Another characteristic of this subline was its enhanced growth after the addition of a pulmonary extract obtained from nude mice. The relationship of these findings to the mechanisms of metastasis of this cancer was discussed.
ISSN:0001-6349
DOI:10.3109/00016348909021015