Structure elucidation of native N- and O-linked glycans by tandem mass spectrometry (tutorial)
Oligosaccharides play important roles in many biological processes. However, the structural elucidation of oligosaccharides remains a major challenge due to the complexities of their structures. Mass spectrometry provides a powerful method for determining oligosaccharide composition. Tandem mass spe...
Saved in:
Published in | Mass spectrometry reviews Vol. 30; no. 4; pp. 560 - 578 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.07.2011
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Oligosaccharides play important roles in many biological processes. However, the structural elucidation of oligosaccharides remains a major challenge due to the complexities of their structures. Mass spectrometry provides a powerful method for determining oligosaccharide composition. Tandem mass spectrometry (MS) provides structural information with high sensitivity. Oligosaccharide structures differ from other polymers such as peptides because of the large number of linkage combinations and branching. This complexity makes the analysis of oligosaccharide unique from that of peptides. This tutorial addresses the issue of spectral interpretation of tandem MS under conditions of collision‐induced dissociation (CID) and infrared multiphoton dissociation (IRMPD). The proper interpretation of tandem MS data can provide important structural information on different types of oligosaccharides including O‐ and N‐linked. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:560–578, 2011 |
---|---|
Bibliography: | ark:/67375/WNG-5CKQ78DM-M ArticleID:MAS20283 istex:E66F9D6CBEFD836605ACE24B2A03731FB5396438 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0277-7037 1098-2787 |
DOI: | 10.1002/mas.20283 |