The poly(A)‐binding protein facilitates in vitro translation of poly(A)‐rich mRNA
To investigate the role of the 73‐kDa poly(A)‐binding protein in protein synthesis, the effect of the addition of homo‐polyribonucleotides on the translation of polyadenylated and non‐adenylated mRNA was studied in the rabbit reticulocyte lysate. Poly(A) was found to be the most effective polynucleo...
Saved in:
Published in | European journal of biochemistry Vol. 176; no. 3; pp. 521 - 526 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.10.1988
Blackwell |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | To investigate the role of the 73‐kDa poly(A)‐binding protein in protein synthesis, the effect of the addition of homo‐polyribonucleotides on the translation of polyadenylated and non‐adenylated mRNA was studied in the rabbit reticulocyte lysate. Poly(A) was found to be the most effective polynucleotide in inhibiting duck‐globin mRNA translation, whereas it had no effect on the translation of polyribosomal duck‐globin mRNP, or on the endogenous synthesis of the rabbit reticulocyte lysate. The translation of poly(A)‐free mRNA was not affected by the addition of poly(A).
Furthermore, we found that the inhibiting effect of poly(A) can be reversed by addition of purified poly(A)‐binding protein. It is thus likely that the 73‐kDa poly(A)‐binding protein is an essential factor necessary for poly(A)‐rich mRNA translation. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0014-2956 1432-1033 |
DOI: | 10.1111/j.1432-1033.1988.tb14309.x |