Mass Spectrometric Immunoassays in Characterization of Clinically Significant Proteoforms

Proteins can exist as multiple proteoforms , as a result of alternative splicing and single-nucleotide polymorphisms (SNPs), as well as posttranslational processing. To address their clinical significance in a context of diagnostic information, proteoforms require a more in-depth analysis. Mass spec...

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Bibliographic Details
Published inProteomes Vol. 4; no. 1; p. 13
Main Authors Trenchevska, Olgica, Nelson, Randall W, Nedelkov, Dobrin
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 17.03.2016
MDPI AG
Subjects
biomarkers
immunoaffinity
mass spectrometry
posttranslational modifications
Review
top-down analysis
top-down analysis
immunoaffinity
mass spectrometry
posttranslational modifications
biomarkers
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Summary:Proteins can exist as multiple proteoforms , as a result of alternative splicing and single-nucleotide polymorphisms (SNPs), as well as posttranslational processing. To address their clinical significance in a context of diagnostic information, proteoforms require a more in-depth analysis. Mass spectrometric immunoassays (MSIA) have been devised for studying structural diversity in human proteins. MSIA enables protein profiling in a simple and high-throughput manner, by combining the selectivity of targeted immunoassays, with the specificity of mass spectrometric detection. MSIA has been used for qualitative and quantitative analysis of single and multiple proteoforms, distinguishing between normal fluctuations and changes related to clinical conditions. This mini review offers an overview of the development and application of mass spectrometric immunoassays for clinical and population proteomics studies. Provided are examples of some recent developments, and also discussed are the trends and challenges in mass spectrometry-based immunoassays for the next-phase of clinical applications.
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ISSN:2227-7382
2227-7382
DOI:10.3390/proteomes4010013