Transcriptome Analysis Revealed the Mechanisms Involved in Ultrasonic Seed Treatment-Induced Aluminum Tolerance in Peanut

Ultrasonic (US) treatment is an efficient method to induce crop tolerance against heavy metal toxicity; however, US-induced aluminum (Al) tolerance in peanuts was rarely studied. This study was comprised of two treatments, namely, CK, without ultrasonic treatment, and US, an ultrasonic seed treatmen...

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Published inFrontiers in plant science Vol. 12; p. 807021
Main Authors Bao, Gegen, Zhou, Qi, Li, Shengyu, Ashraf, Umair, Huang, Suihua, Miao, Aimin, Cheng, Zhishang, Wan, Xiaorong, Zheng, Yixiong
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 08.02.2022
Subjects
aluminum
gene expression
growth
peanut
Plant Science
transcription factor genes
growth
peanut
aluminum
transcription factor genes
gene expression
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Summary:Ultrasonic (US) treatment is an efficient method to induce crop tolerance against heavy metal toxicity; however, US-induced aluminum (Al) tolerance in peanuts was rarely studied. This study was comprised of two treatments, namely, CK, without ultrasonic treatment, and US, an ultrasonic seed treatment, for 15 min. Both treated and non-treated treatments were applied with Al in the form of AlCl .18H O at 5 mmol L in Hoagland solution at one leaf stage. Results depicted that plant height, main root length, and number of lateral roots increased significantly under US treatment. Transcriptome analysis revealed that plant hormone signal transduction and transcription factors (TFs) were significantly enriched in the differentially expressed genes (DEGs) in US treatment, and the plant hormones were measured, including salicylic acid (SA) and abscisic acid (ABA) contents, were substantially increased, while indole acetic acid (IAA) and jasmonic acid (JA) contents were decreased significantly in US treatment. The TFs were verified using quantitative real-time (qRT)-PCR, and it was found that multiple TFs genes were significantly upregulated in US treatment, and and genes were also significantly upregulated in US treatment. Overall, the US treatment induced the regulation of hormone content and regulated gene expression by regulating TFs to improve Al tolerance in peanuts. This study provided a theoretical rationale for US treatment to improve Al tolerance in peanuts.
Bibliography:Reviewed by: Deepak Kumar Verma, Indian Institute of Technology Kharagpur, India; Muhammad Azhar Nadeem, Sivas University of Science and Technology, Turkey; Basharat Ali, University of Agriculture, Faisalabad, Pakistan
Edited by: Rafaqat Ali Gill, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences (CAAS), China
This article was submitted to Plant Nutrition, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2021.807021