Subpopulation of Macrophage-Like Plasmatocytes Attenuates Systemic Growth via JAK/STAT in the Drosophila Fat Body

• Published in: Frontiers in immunology Vol. 11; p. 63
• Main Authors: Shin, Mingyu, Cha, Nuri, Koranteng, Ferdinand, Cho, Bumsik, Shim, Jiwon
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 31.01.2020
• Subjects: Animals; Drosophila melanogaster; Drosophila melanogaster - cytology; Drosophila melanogaster - physiology; Drosophila Proteins - metabolism; Fat Body - metabolism; Growth - physiology; Hemocytes - cytology; Hemocytes - physiology; Hemolectin; Immunology; insulin signaling; JAK/STAT; Janus Kinases - metabolism; Larva; Macrophages - physiology; plasmatocytes; Signal Transduction; STAT Transcription Factors - metabolism; Transcription Factors - metabolism; upd3; insulin signaling; plasmatocytes; Hemolectin; JAK/STAT; upd3; Drosophila melanogaster; Peroxidasin
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2020.00063

hemocytes, like those of mammals, are given rise from two distinctive phases during both the embryonic and larval hematopoiesis. Embryonically derived hemocytes, mostly composed of macrophage-like plasmatocytes, are largely identified by genetic markers. However, the cellular diversity and distinct functions of possible subpopulations within plasmatocytes have not been explored in larvae. Here, we show that larval plasmatocytes exhibit differential expressions of and during development. Moreover, removal of plasmatocytes by overexpressing pro-apoptotic genes, and in -positive plasmatocytes, feeding high sucrose diet, or wasp infestation results in increased circulating hemocytes that are -negative. Interestingly these -negative plasmatocytes retain expression, and animals expressing -negative and -positive subtype largely attenuate growth and abrogate metabolism. Furthermore, elevated levels of a cytokine, , are detected when -positive hemocytes are ablated, which in turn activates JAK/STAT activity in several tissues including the fat body. Finally, we observed that insulin signaling is inhibited in this background, which can be recovered by concurrent loss of . Overall, this study highlights heterogeneity in plasmatocytes and a functional plasticity of each subtype, which reaffirms extension of their role beyond immunity into metabolic regulation for cooperatively maintaining internal homeostatic balance.