Salmonella enterica serovar Infantis from Food and Human Infections, Switzerland, 2010-2015: Poultry-Related Multidrug Resistant Clones and an Emerging ESBL Producing Clonal Lineage

• Published in: Frontiers in microbiology Vol. 8; p. 1322
• Main Authors: Hindermann, Denise, Gopinath, Gopal, Chase, Hannah, Negrete, Flavia, Althaus, Denise, Zurfluh, Katrin, Tall, Ben D, Stephan, Roger, Nüesch-Inderbinen, Magdalena
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 13.07.2017
• Subjects: blaCTX-M-65; food; humans; Hungarian clone B; Microbiology; Salmonella Infantis; Salmonella Infantis; blaCTX-M-65; humans; Hungarian clone B; food
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2017.01322

The aim of this study was to characterize a collection of 520 serovar Infantis strains isolated from food (poultry meat), human infections and environmental sources from the years 2010, 2013 and 2015 in Switzerland. We performed antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE) analysis on all 520 . Infantis isolates, and whole genome sequencing (WGS) on 32 selected isolates. The majority (74.8%) of the isolates was multidrug resistant (MDR). PFGE analysis revealed that 270 (51.9%) isolates shared an identity of 90%. All isolates subjected to WGS belonged to sequence type (ST) 32 or a double-locus variant thereof (one isolate). Seven (21.9%) of the sequenced isolates were phylogenetically related to the broiler-associated clone B that emerged in Hungary and subsequently spread within and outside of Europe. In addition, three isolates harboring on a predicted large (∼320 kb) plasmid grouped in a distinct cluster. This study documents the presence of the Hungarian clone B and related clones in food and human isolates between 2010 and 2015, and the emergence of a harboring MDR . serovar Infantis lineage.