Increased vincristine production from Agrobacterium tumefaciens C58 induced shooty teratomas of Catharanthus roseus G. Don

Dimeric indole alkaloids are used extensively for cancer therapy. Agrobacterium tumefaciens C58 strain was used for induction of shooty teratoma in Catharanthus roseus using epicotyl and stem node explants. The transformed nature of shooty teratomas was confirmed by nopaline assay. Growth kinetics o...

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Published inNatural product research Vol. 23; no. 11; pp. 973 - 981
Main Authors Begum, Fathimunnisa, Nageswara Rao, S.S.S., Rao, Kiranmayee, Prameela Devi, Y., Giri, A., Giri, C.C.
Format Journal Article
LanguageEnglish
Published England Taylor & Francis Group 01.01.2009
Subjects
Agrobacterium tumefaciens
Agrobacterium tumefaciens - genetics
Catharanthus - genetics
Catharanthus - metabolism
Catharanthus - microbiology
Catharanthus roseus
Catharanthus roseus G. Don
dimeric alkaloid
nopaline
Plant Tumors - microbiology
shooty teratomas
Transformation, Genetic
vincristine
Vincristine - biosynthesis
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Summary:Dimeric indole alkaloids are used extensively for cancer therapy. Agrobacterium tumefaciens C58 strain was used for induction of shooty teratoma in Catharanthus roseus using epicotyl and stem node explants. The transformed nature of shooty teratomas was confirmed by nopaline assay. Growth kinetics of shooty teratomas depicted maximum growth during 21-24 days of culture. Dimeric alkaloid vincristine in the transformed cultures was present at a concentration of 0.011 that was tenfold higher compared to untransformed control cultures.
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ISSN:1478-6419
1478-6427
DOI:10.1080/14786410802131153