Protein synthesis in isolated mitochondria of rice (Oryza sativa L.) seedlings

• Published in: Plant physiology (Bethesda) Vol. 96; no. 1; pp. 319 - 323
• Main Authors: Dai, H, Lo, Y S, Wu, C Y, Tsou, C L, Hsu, G S, Chern, C G, Ruddat, M, Chiang, K S
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.05.1991
• Subjects: Bacteria; Bacterial contamination; biodegradacion; biodegradation; Biological and medical sciences; Communications; esterilizacion; Fundamental and applied biological sciences. Psychology; Grains; infeccion; infection; Metabolism; Mitochondria; mitochondrie; mitocondria; Nitrogen metabolism; oryza sativa; Plant physiology and development; Plants; plantulas; plantule; Protein synthesis; Rice; Seedlings; sintesis de proteinas; Sodium; sterilisation germes; Sterilization; sterilizing; synthese proteique; Proteins; Oryza sativa; Monocotyledones; Mitochondria; Purification; Radiolabelling; Gramineae; Protein synthesis; Angiospermae; Isolation; Spermatophyta; Cereal crop
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.96.1.319

For studies of in organello mitochondrial protein synthesis in rice, Oryza sativa L., conventional surface-sterilization procedures were demonstrated to be ineffective. Because of the over-whelmingly efficient [35S]methionine utilization by contaminating bacteria, even "essentially bacteria-free" rice mitochondria were shown to be unsuitable for the study of in organello protein synthesis. We developed a procedure to obtain a bacteria-free preparation of rice mitochondria. Such mitochondria favored a membrane-dependent ATP-generating system over an external ATP-generating system as the energy supplement for in organello protein synthesis. Two distinct classes of [35S]methionine-labeled, cycloheximide-insensitive products were detected: an electrophoretically unresolved population and a set of some 22 to 27 discrete polypeptide species, each with a characteristic electrophoretic mobility and relative abundance.