Generation of human induced pluripotent stem cell line (WMUi001-A) from a patient with aortic dissection

• Published in: Stem cell research Vol. 43; p. 101730
• Main Authors: Jin, Peifeng, Wang, Sixian, Jiang, Xu, Shan, Xiaoqiong, Jiang, Sheng, Quan, Yingyi, Zhang, Tianci, Yang, Jiwen, Zhang, Huang, Ma, Jianshe, Dong, Miaowu, Fan, Junming, Fan, Xiaofang, Gong, Yongsheng, Wang, Yongyu
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.03.2020; Elsevier
• Subjects: Aneurysm, Dissecting - genetics; Cell Line - metabolism; Humans; Induced Pluripotent Stem Cells - metabolism; Male; Middle Aged
• ISSN: 1873-5061; 1876-7753
• DOI: 10.1016/j.scr.2020.101730

A human induced pluripotent stem cell (hiPSC) line (WMUi001-A) was generated from the aortic tissue of a 47-year-old donor with aortic dissection and normal blood pressure. Integration-free episomal vector-mediated reprogramming was used for the generation of this iPSC line. The established iPSC line was found to express pluripotency markers, exhibit a differentiation potential in vitro, as well as display a normal karyotype. We further identified that this iPSC line contained a mutation in collagen type IV (COL4A2, R131M), which may serve as a useful tool for the disease modeling of aortic dissection.