Comparative Genomics Reveals Evidence of Genome Reduction and High Extracellular Protein Degradation Potential in Kangiella

• Published in: Frontiers in microbiology Vol. 9; p. 1224
• Main Authors: Wang, Jiahua, Lu, Ye, Nawaz, Muhammad Z, Xu, Jun
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 07.06.2018
• Subjects: genome reduction; Kangiella; marine bacteria; Microbiology; Oceanospirillales; protein degradation; Kangiella; marine bacteria; genome reduction; Oceanospirillales; protein degradation
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2018.01224

The genus has recently been proposed within the family Kangiellaceae, belonging to order Oceanospirillales. Here, we report the complete genome sequence of a novel strain, FT102, which is the only species isolated from a deep sea sediment sample. Furthermore, gaps in the publicly available genome scaffold of DSM 16071 (NCBI Reference Sequence: NZ_ARFE00000000.1) were also filled using polymerase chain reaction (PCR) and Sanger sequencing. A comparative genomic analysis of five and 18 non- strains revealed insights into their metabolic potential. It was shown that low genomic redundancy and -lineage-specific gene loss are the key reasons behind the genome reduction in compared to that in any other free-living Oceanospirillales strain. The occurrence of relatively diverse and more frequent extracellular protease-coding genes along with the incomplete carbohydrate metabolic pathways in the genome suggests that has high extracellular protein degradation potential. Growth of strains has been observed using amino acids as the only carbon and nitrogen source and tends to increase with additional tryptone. Here, we propose that extracellular protein degradation and amino acid utilization are significant and prominent features of . Our study provides more insight into the genomic traits and proteolytic metabolic capabilities of .