Decoding the Plant Growth Promotion and Antagonistic Potential of Bacterial Endophytes From Ocimum sanctum Linn. Against Root Rot Pathogen Fusarium oxysporum in Pisum sativum

• Published in: Frontiers in plant science Vol. 13; p. 813686
• Main Authors: Gupta, Shikha, Pandey, Sangeeta, Sharma, Satyawati
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 14.02.2022
• Subjects: biological control; endophytes; Fusarium root rot; induced systemic resistance; plant growth promotion; Plant Science; volatile compounds; induced systemic resistance; volatile compounds; Fusarium root rot; biological control; plant growth promotion; endophytes
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.813686

The present study demonstrates plant growth promotion and induction of systemic resistance in pea ( ) plant against f.sp. by two bacterial endophytes, OS_12 and OS_25 isolated from leaves of Linn. The endophytes were evaluated for their antagonistic potential against three phytopathogens , f. sp. , and by dual culture assay. Maximum inhibition of f. sp. was observed by strains OS_12 and OS_25 among all root rot pathogens. Scanning electron microscopy of dual culture indicated hyphal distortion and destruction in the case of f. sp. . Further, volatile organic compounds (VOCs) were identified by gas chromatography-mass spectrometry (GC-MS). The GC-MS detected eight bioactive compounds from hexane extracts for instance, Dodecanoic acid, Tetra decanoic acid, L-ascorbic acid, -13-Octadecanoic acid, Octadecanoic acid. Both the endophytes exhibited multifarious plant growth promoting traits such as indole acetic production (30-33 μg IAA ml ), phosphate solubilization, and siderophore and ammonia production. Pot trials were conducted to assess the efficacy of endophytes in field conditions. A significant reduction in disease mortality rate and enhancement of growth parameters was observed in pea plants treated with consortium of endophytes OS_12 and OS_25 challenged with f.sp. infection. The endophytic strains elicited induced systemic resistance (ISR) in pathogen challenged pea plants by enhancing activities of Phenylalanine ammonia lyase (PAL), peroxidase (PO), polyphenol oxidase (PPO), ascorbate oxidase (AO), catalase (CAT) and total phenolic content. The endophytes reduced the oxidative stress as revealed by decrease in malondialdehyde (MDA) content and subsequently, lipid peroxidation in host plant leaves. Robust root colonization of pea seedlings by endophytes was observed by scanning electron microscopy (SEM) and fluorescence microscopy. Thus, plant growth promoting endophytic and can be further exploited through bio-formulations for sustainable protection of crops against root rot diseases as bio-control agents.