Integrated analysis reveals effects of bioactive ingredients from Limonium Sinense (Girard) Kuntze on hypoxia-inducible factor (HIF) activation

• Published in: Frontiers in plant science Vol. 13; p. 994036
• Main Authors: Zhao, Hualong, Wang, Siyuan, Zhou, Yilu, Ertay, Ayse, Williamson, Philip T. F., Ewing, Rob M., Tang, Xinhui, Wang, Jialian, Wang, Yihua
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 27.10.2022
• Subjects: cell cycle; cell viability; gallic acid; hypoxia-inducible factor; Limonium Sinense (Girard) Kuntze; Plant Science; Limonium Sinense (Girard) Kuntze; hypoxia-inducible factor; cell viability; cell cycle; gallic acid
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.994036

Limonium Sinense (Girard) Kuntze is a traditional Chinese medicinal herb, showing blood replenishment, anti-tumour, anti-hepatitis, and immunomodulation activities amongst others. However, the mechanism of its pharmacological activities remains largely unknown. Here, we investigated the effects of bioactive ingredients from Limonium Sinense using an integrated approach. Water extracts from Limonium Sinense (LSW) showed a strong growth inhibitory effect on multiple cells in both 2D and 3D cultures. Global transcriptomic profiling and further connectivity map (CMap) analysis identified several similarly acting therapeutic candidates, including Tubulin inhibitors and hypoxia-inducible factor (HIF) modulators. The effect of LSW on the cell cycle was verified with flow cytometry showing a G2/M phase arrest. Integrated analysis suggested a role for gallic acid in mediating HIF activation. Taken together, this study provides novel insights into the bioactive ingredients in Limonium Sinense , highlighting the rich natural resource and therapeutic values of herbal plants.