Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study

Oral squamous cell carcinoma (OSCC) is a devastating disease that is usually associated with a dense associated inflammatory infiltrate. Characterizing tumor-associated inflammation is critical to understand the pathogenies of tumor development and progression. We have tested a protocol to analyze t...

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Published inFrontiers in oral health Vol. 2; p. 740469
Main Authors Laliberté, Catherine, Ng, Nicole, Eymael, Denise, Higgins, Kevin, Ali, Aiman, Kiss, Alex, Bradley, Grace, Magalhaes, Marco A O
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 21.09.2021
Subjects
cancer
cytokine
dysplasia
inflammation
oral cancer
Oral Health
saliva
cytokine
dysplasia
saliva
IL6
chemokine
inflammation
cancer
oral cancer
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Summary:Oral squamous cell carcinoma (OSCC) is a devastating disease that is usually associated with a dense associated inflammatory infiltrate. Characterizing tumor-associated inflammation is critical to understand the pathogenies of tumor development and progression. We have tested a protocol to analyze tissue and salivary immune cells and mediators of 37 patients with OSCC at different stages and compared to eight chronic periodontitis patients and 24 healthy controls. Tissue analysis was based on fluorescent immunohistochemistry (FIHC) and inflammatory mediators were analyzed using a Luminex-based 30-Plex panel. Immune cells were analyzed using multichannel flow cytometry including CD45, CD66b, CD3, CD4, CD8, CD25, CD56, CD68, CD138, PD-1, and PD-L1. We show an increase in OSCC-associated inflammation characterized by increased pro-inflammatory cytokines including IL-6, IL-8, TNFα, and GMCSF and increased salivary immune cells. We described a new method to analyze salivary inflammatory markers that can be used in future studies to monitor disease progression and prognosis.
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This article was submitted to Oral Cancers, a section of the journal Frontiers in Oral Health
Edited by: Anthony Faber, Virginia Commonwealth University, United States
Reviewed by: Sven E. Niklander, Universidad Andres Bello, Chile; Brendan Conn, University of Edinburgh, United Kingdom
ISSN:2673-4842
2673-4842
DOI:10.3389/froh.2021.740469