Nicotinamide Phosphate Transferase (NAMPT) Increases in Plasma in Patients with Acute Coronary Syndromes, and Promotes Macrophages to M2 Polarization

• Published in: International Heart Journal Vol. 59; no. 5; pp. 1116 - 1122
• Main Authors: Zhang, Chengxin, Zhu, Rui, Wang, Huiping, Tao, Qianshan, Lin, Xianhe, Ge, Shenglin, Zhai, Zhimin
• Format: Journal Article
• Language: English
• Published: Japan International Heart Journal Association 01.09.2018; Japan Science and Technology Agency
• Subjects: Acute coronary syndromes; Arteriosclerosis; Atherosclerosis; Cell culture; Inflammatory diseases; Interleukin 1 receptor antagonist; Interleukin 1 receptors; Interleukin 10; Interleukin 13; Interleukin 4; Interleukin 6; Macrophage polarization; Macrophages; Monocytes; NAD; NADPH; Nicotinamide; Peripheral blood mononuclear cells; Polarization; Signal transduction; SIRT1 protein; Macrophage polarization; Atherosclerosis
• ISSN: 1349-2365; 1349-3299
• DOI: 10.1536/ihj.17-363

Atherosclerosis is an inflammatory disease; monocytes and macrophages play an important role in the progression of this disease. However, the mechanisms are not fully understood yet. Nicotinamide phosphate transferase (NAMPT) is the rate limiting enzyme in the synthesis of NAD, but extracellular NAMPT shows the characteristics of cytokines/adipokines, suggesting that it may be a link between metabolism and inflammation. In this study, we compared the expression levels of the NAMPT/NAD+/Sirt1 signaling pathway as well as NAMPT, CRP and IL-6 in the peripheral blood mononuclear cell (PBMC), and plasma in patients with acute coronary syndromes and healthy subjects, and analyzed their association with macrophage polarization. The relationship between eNAMPT and iNAMPT and the polarization of macrophages was analyzed by NAD+, NAMPT blocker, and neutralizing antibody treatment. The results showed that the expression of the NAMPT/NAD+/Sirt1 signaling pathway was up-regulated in the peripheral blood of patients with ACS. Inhibition of iNAMPT expression can reduce M1 polarization; however, there was no significant effect on eNAMPT secretion and M2 polarization. Neutralizing eNAMPT by neutralizing antibodies can reduce M2 polarization and decrease the expression levels of IL-10, IL-13, IL-4 and IL-1ra. The addition of NAD+ in the cell culture supernatant had no significant effect on the polarization of M1 but increased the M2 polarization and the expression levels of IL-10 and IL-1ra. Our findings suggested that NAMPT is involved in the pathogenesis of atherosclerosis; the increased expression of eNAMPT in ACS patients may play a protective role by the up regulation of the NAMPT/NAD+/Sirt1 signaling pathway.