Telomeric DNA-RNA-hybrid G-quadruplex exists in environmental conditions of HeLa cells

• Published in: Chemical communications (Cambridge, England) Vol. 56; no. 48; pp. 6547 - 655
• Main Authors: Bao, Hong-Liang, Xu, Yan
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 18.06.2020
• Subjects: Animals; chemical compounds; chemical reactions; Deoxyribonucleic acid; Dichroism; DNA; DNA - chemistry; environmental factors; fluorine; G-Quadruplexes; HeLa Cells; human cell lines; Humans; Magnetic Resonance Spectroscopy; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Nucleic Acid Conformation; Oocytes - metabolism; Ribonucleic acid; RNA; RNA - chemistry; stable isotopes; Telomere - chemistry; telomeres; Xenopus laevis - genetics; Xenopus laevis - growth & development; Xenopus laevis - metabolism

In the present study, we employed a 19 F NMR approach to study the association of telomere RNA and DNA in vitro and in living human cells. We successfully characterized the DNA-RNA hybrid G-quadruplex (HQ) structure formed by telomeric DNA and RNA. We further demonstrated for the first time that an...