Telomeric DNA-RNA-hybrid G-quadruplex exists in environmental conditions of HeLa cells

In the present study, we employed a 19 F NMR approach to study the association of telomere RNA and DNA in vitro and in living human cells. We successfully characterized the DNA-RNA hybrid G-quadruplex (HQ) structure formed by telomeric DNA and RNA. We further demonstrated for the first time that an...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 56; no. 48; pp. 6547 - 655
Main Authors Bao, Hong-Liang, Xu, Yan
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 18.06.2020
Subjects
Animals
chemical compounds
chemical reactions
Deoxyribonucleic acid
Dichroism
DNA
DNA - chemistry
environmental factors
fluorine
G-Quadruplexes
HeLa Cells
human cell lines
Humans
Magnetic Resonance Spectroscopy
NMR
Nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
Nucleic Acid Conformation
Oocytes - metabolism
Ribonucleic acid
RNA
RNA - chemistry
stable isotopes
Telomere - chemistry
telomeres
Xenopus laevis - genetics
Xenopus laevis - growth & development
Xenopus laevis - metabolism
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Summary:In the present study, we employed a 19 F NMR approach to study the association of telomere RNA and DNA in vitro and in living human cells. We successfully characterized the DNA-RNA hybrid G-quadruplex (HQ) structure formed by telomeric DNA and RNA. We further demonstrated for the first time that an HQ conformation can exist in the environmental conditions of HeLa cells. In the present study, we employed a 19 F NMR approach to study the association of telomere RNA and DNA in vitro and in living human cells.
Bibliography:Electronic supplementary information (ESI) available: RNA synthesis, circular dichroism measurements, melting curve measurements, NMR measurements, Fig. S1-S12, and Table S1. See DOI
10.1039/d0cc02053b
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ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d0cc02053b