The Loss of Function of the NODULE INCEPTION-Like PROTEIN 7 Enhances Salt Stress Tolerance in Arabidopsis Seedlings

• Published in: Frontiers in plant science Vol. 12; p. 743832
• Main Authors: Le, Quang Tri, Lee, Won Je, Choi, Jun Ho, Nguyen, Dinh Thanh, Truong, Hai An, Lee, Sang-A, Hong, Suk-Whan, Lee, Hojoung
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 24.01.2022
• Subjects: Arabidopsis; nitrate reductase; nitric oxide; NLP7; Plant Science; salt stress tolerance; salt stress tolerance; NLP7; nitric oxide; nitrate reductase; Arabidopsis
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2021.743832

Plants acquire nitrogen, an essential macronutrient, from the soil as nitrate. Since nitrogen availability is a major determinant of crop productivity, the soil is amended with nitrogenous fertilizers. Extensive use of irrigation can lead to the accumulation of salt in the soil, which compromises crop productivity. Our characterization of ( ), a transcription factor regulating the primary response to nitrate, revealed an intersection of salt stress and nitrate metabolism. The growth of loss-of-function mutant was tolerant to high salinity that normally reduces the fresh weight and chlorophyll and protein content of wild type (Col-0). On a medium with high salinity, the experienced less stress, accumulating less proline, producing less nitric oxide (NO) and reactive oxygen species (ROS), and expressing lower transcript levels of marker genes, such as and , than Col-0. Nevertheless, more sodium ions were translocated to and accumulated in the shoots of than that of Col-0. Since also expressed less nitrate reductase (NR) activity, nitrate accumulated to abnormally high levels with or without salinity. We attributed the enhanced salt tolerance of to the balanced accumulation of nitrate anions and sodium cations. Our results suggest that nitrate metabolism and signaling might be targeted to improve salt tolerance.