Unraveling Assemblage, Functions and Stability of the Gut Microbiota of Blattella germanica by Antibiotic Treatment

Symbiosis between prokaryotes and eukaryotes is a widespread phenomenon that has contributed to the evolution of eukaryotes. In cockroaches, two types of symbionts coexist: an endosymbiont in the fat body ( ), and a rich gut microbiota. The transmission mode of is vertical, while the gut microbiota...

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Published inFrontiers in microbiology Vol. 11; p. 487
Main Authors Domínguez-Santos, Rebeca, Pérez-Cobas, Ana Elena, Artacho, Alejandro, Castro, José A., Talón, Irene, Moya, Andrés, García-Ferris, Carlos, Latorre, Amparo
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 25.03.2020
Subjects
antibiotics
Blattella germanica
endosymbiosis
functional resilience
gut microbiota
Microbiology
functional resilience
Blattella germanica
antibiotics
endosymbiosis
gut microbiota
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Summary:Symbiosis between prokaryotes and eukaryotes is a widespread phenomenon that has contributed to the evolution of eukaryotes. In cockroaches, two types of symbionts coexist: an endosymbiont in the fat body ( ), and a rich gut microbiota. The transmission mode of is vertical, while the gut microbiota of a new generation is mainly formed by bacterial species present in feces. We have carried out a metagenomic analysis of populations, treated and non-treated with two antibiotics (vancomycin and ampicillin) over two generations to (1) determine the core of bacterial communities and potential functions of the gut microbiota and (2) to gain insights into the mechanisms of resistance and resilience of the gut microbiota. Our results indicate that the composition and functions of the bacteria were affected by treatment, more severely in the case of vancomycin. Further results demonstrated that in an untreated second-generation population that comes from antibiotic-treated first-generation, the microbiota is not yet stabilized at nymphal stages but can fully recover in adults when feces of a control population were added to the diet. This signifies the existence of a stable core in either composition and functions in lab-reared populations. The high microbiota diversity as well as the observed functional redundancy point toward the microbiota of cockroach hindguts as a robust ecosystem that can recover from perturbations, with recovery being faster when feces are added to the diet.
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Edited by: Michael Poulsen, University of Copenhagen, Denmark
Reviewed by: Alexander V. Tyakht, Institute of Gene Biology (RAS), Russia; Katie Lynn Summers, United States Department of Agriculture (USDA), United States
Present address: Irene Talón, Department of Development and Regeneration, Leuven Stem Cell Institute, KU Leuven, Leuven, Belgium
These authors have contributed equally to this work
This article was submitted to Microbial Symbioses, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.00487