Characterization of genes encoding proteins containing HD-related output domain in Xanthomonas campestris pv. campestris

• Published in: Antonie van Leeuwenhoek Vol. 109; no. 4; pp. 509 - 522
• Main Authors: Lee, Hsien-Ming, Liao, Chao-Tsai, Chiang, Ying-Chuan, Chang, Yu-Yin, Yeh, Yu-Tzu, Du, Shin-Chiao, Hsiao, Yi-Min
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.04.2016; Springer Nature B.V
• Subjects: Amino Acid Sequence; Bacteria; bacterial adhesion; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Biochemistry; Biomedical and Life Sciences; Brassicaceae; cell movement; Gene Expression Regulation, Bacterial; Genes; Genome, Bacterial; Life Sciences; Medical Microbiology; Microbiology; Mutation; Original Paper; plant pathogens; Plant Sciences; Plants - microbiology; Proteins; Sequence Alignment; Soil Science & Conservation; stress response; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; virulence; Virulence Factors - biosynthesis; Virulence Factors - genetics; Xanthomonas campestris; Xanthomonas campestris - genetics; Xanthomonas campestris pv. campestris; Transcriptional regulation; Bacterial attachment; Xanthomonas
• ISSN: 0003-6072; 1572-9699; 1572-9699
• DOI: 10.1007/s10482-016-0656-y

The Gram-negative plant pathogen Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers. The production of Xcc virulence factors is regulated by Clp and RpfF. HD-related output domain (HDOD) is a protein domain of unknown biochemical function. The genome of Xcc encodes three proteins (GsmR, HdpA, and HdpB) with an HDOD. The GsmR has been reported to play a role in the general stress response and cell motility and its expression is positively regulated by Clp. Here, the function and transcription of hdpA and hdpB were characterized. Mutation of hdpA resulted in enhanced bacterial attachment. In addition, the expression of hdpA was positively regulated by RpfF but not by Clp, subject to catabolite repression and affected by several stress conditions. However, mutational analysis and reporter assay showed that hdpB had no effect on the production of a range of virulence factors and its expression was independent of Clp and RpfF. The results shown here not only extend the previous work on RpfF regulation to show that it influences the expression of hdpA in Xcc, but also expand knowledge of the function of the HDOD containing proteins in bacteria.