Expression, characterization and therapeutic efficacy of chimeric Fab of anti-idiotypic antibody NP30 against Schistosoma japonicum
► A human–mouse chimeric Fab of monoclonal anti-idiotypic antibody NP30 (chFab-NP30) against Schistosoma japonicum was constructed and expressed in E. coli. ► The purified chFab-NP30 inhibited the binding of parent NP30 to sera from schistosomiasis patients in a dose-dependent manner. ► Administrati...
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Published in | Acta tropica Vol. 118; no. 2; pp. 159 - 164 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier B.V
01.05.2011
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | ► A human–mouse chimeric Fab of monoclonal anti-idiotypic antibody NP30 (chFab-NP30) against Schistosoma japonicum was constructed and expressed in E. coli. ► The purified chFab-NP30 inhibited the binding of parent NP30 to sera from schistosomiasis patients in a dose-dependent manner. ► Administration of the chimeric Fab in vivo increased the survival rate, reduced egg burdens and ameliorated organ pathology of mice with acute schistosomiasis. ► chFab-NP30 is a promising candidate to be used as a specific and efficient recombinant antibody against acute schistosomiasis japonica.
The murine monoclonal anti-idiotypic antibody NP30 is a promising therapeutic antibody against Schistosoma japonicum. However, the immunogenicity of murine NP30 limits its further study and application in humans. Here the chimeric Fab of NP30 (chFab-NP30) comprising the variable regions of murine NP30 and constant regions of human antibody was assembled. chFab-NP30 was expressed and purified as a soluble and functional protein. Administration of chFab-NP30 in vivo increased the survival rate, reduced egg burdens and ameliorated organ pathology of mice with acute schistosomiasis. Our study indicated that chFab-NP30 is a promising candidate to be used as a specific and efficient recombinant antibody against acute schistosomiasis japonica. Further studies on function mechanism of chFab-NP30 needs to be carried out in the future. |
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Bibliography: | http://dx.doi.org/10.1016/j.actatropica.2011.01.006 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0001-706X 1873-6254 |
DOI: | 10.1016/j.actatropica.2011.01.006 |