Expression, characterization and therapeutic efficacy of chimeric Fab of anti-idiotypic antibody NP30 against Schistosoma japonicum

• Published in: Acta tropica Vol. 118; no. 2; pp. 159 - 164
• Main Authors: Xu, Jing, Zhu, Xiao-Juan, Li, Yu-Hua, Dai, Yang, Zhu, Yin-Chang, Zheng, Jiang, Feng, Zhen-Qing, Guan, Xiao-Hong
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 01.05.2011; Elsevier
• Subjects: Animals; Anthelmintics - administration & dosage; Anthelmintics - isolation & purification; Anti-idiotypic antibody; Antibodies, Anti-Idiotypic - administration & dosage; Antibodies, Anti-Idiotypic - genetics; Antibodies, Anti-Idiotypic - isolation & purification; Antibodies, Monoclonal - administration & dosage; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - isolation & purification; Biological and medical sciences; Chimeric Fab; chimerism; Diseases caused by trematodes; eggs; General aspects; Helminthic diseases; humans; immune response; Infectious diseases; Medical sciences; Mice; Mice, Inbred BALB C; Parasite Egg Count; Parasitic diseases; Portal Vein - parasitology; Recombinant Proteins - administration & dosage; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Schistosoma japonicum; Schistosoma japonicum - drug effects; Schistosomiases; Schistosomiasis; Schistosomiasis japonica - drug therapy; Schistosomiasis japonica - parasitology; survival rate; Therapeutic efficacy; Therapeutic efficacy; Schistosomiasis; Chimeric Fab; Anti-idiotypic antibody; Infection; Treatment; Antibody; Trematode disease; Tropical medicine; Parasitosis; Helminthiasis
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2011.01.006

► A human–mouse chimeric Fab of monoclonal anti-idiotypic antibody NP30 (chFab-NP30) against Schistosoma japonicum was constructed and expressed in E. coli. ► The purified chFab-NP30 inhibited the binding of parent NP30 to sera from schistosomiasis patients in a dose-dependent manner. ► Administration of the chimeric Fab in vivo increased the survival rate, reduced egg burdens and ameliorated organ pathology of mice with acute schistosomiasis. ► chFab-NP30 is a promising candidate to be used as a specific and efficient recombinant antibody against acute schistosomiasis japonica. The murine monoclonal anti-idiotypic antibody NP30 is a promising therapeutic antibody against Schistosoma japonicum. However, the immunogenicity of murine NP30 limits its further study and application in humans. Here the chimeric Fab of NP30 (chFab-NP30) comprising the variable regions of murine NP30 and constant regions of human antibody was assembled. chFab-NP30 was expressed and purified as a soluble and functional protein. Administration of chFab-NP30 in vivo increased the survival rate, reduced egg burdens and ameliorated organ pathology of mice with acute schistosomiasis. Our study indicated that chFab-NP30 is a promising candidate to be used as a specific and efficient recombinant antibody against acute schistosomiasis japonica. Further studies on function mechanism of chFab-NP30 needs to be carried out in the future.