Aggregatibacter actinomycetemcomitans Outer Membrane Proteins 29 and 29 Paralogue Induce Evasion of Immune Response

• Published in: Frontiers in oral health Vol. 3; p. 835902
• Main Authors: da Silva, Maike Paulino, Silva, Viviam de Oliveira, Pasetto, Silvana, Ando-Suguimoto, Ellen Sayuri, Kawamoto, Dione, Mata, Gardênia Márcia Silva Campos, Murata, Ramiro Mendonça, Mayer, Marcia Pinto Alves, Chen, Casey
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 03.02.2022
• Subjects: Aggregatibacter actinomycetemcomitans; aggressive periodontitis; gingival epithelial cells; inflammatory response; Oral Health; outer membrane protein 29; outer membrane protein 29 paralogue; outer membrane protein 29; Aggregatibacter actinomycetemcomitans; outer membrane protein 29 paralogue; gingival epithelial cells; inflammatory response; aggressive periodontitis; virulence factors
• ISSN: 2673-4842; 2673-4842
• DOI: 10.3389/froh.2022.835902

( ) is abundant within the microbial dysbiotic community of some patients with periodontitis. outer membrane protein 29 (OMP29), a member of the OMPA family, mediates the invasion of to gingival epithelial cells (GECs). This study evaluated the effect of OMP29 and its paralogue OMP29 on the response of GECs to . The or/and deletion mutants AaΔ29, AaΔ29P, and AaΔ29Δ29P were constructed, and recombinant OMP29 was obtained. Microarray analysis and the evaluation of gene expression were performed to examine the response of GECs line OBA-09 to and its mutants. The expression of and its product CXCL-8 was examined in LPS-stimulated OBA-09 cells with OMP29 . Proteomics analysis showed that the deletion of led to overexpression of both OMP29 and another membrane protein OMP39, the expression of which was further increased in AaΔ29Δ29P. OBA-09 cells challenged with AaΔ29Δ29P exhibited a higher expression of in comparison to wildtype strain AaD7S or single-deletion mutants AaΔ29 or AaΔ29P. LPS-stimulated OBA-09 cells challenged with OMP29 showed reduced expressions of and its product CXCL-8. OBA-09 cells challenged with AaΔ29Δ29P in comparison to strain AaD7S resulted in higher expressions of genes involved in apoptosis and inflammatory response such as α β , and β and reduced expressions of , and . The results suggested a novel strategy of , mediated by OMP29 and OMP29 , to evade host immune response by inhibiting CXCL-8 expression and modulating the genes involved in apoptosis and inflammatory response in GECs. Pending further confirmation, the strategy might interfere with the recruitment of neutrophils and dampen the host inflammatory response, leading to a more permissive subgingival niche for bacterial growth.