In vitro Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis
Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, and molecular aspects can affect cell motility is a challenge to understand migratory cells b...
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Published in | Frontiers in cell and developmental biology Vol. 7; p. 107 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
14.06.2019
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Subjects | |
Online Access | Get full text |
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Summary: | Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, and molecular aspects can affect cell motility is a challenge to understand migratory cells behavior.
assays are excellent approaches to extrapolate to
situations and study live cells behavior. Here we present four
protocols that describe step-by-step cell migration, invasion and adhesion strategies and their corresponding image data quantification. These current protocols are based on
wound healing assays (comparing traditional pipette tip-scratch assay vs. culture insert assay), 2D individual cell-tracking experiments by live cell imaging and
spreading and transwell assays. All together, they cover different phenotypes and hallmarks of cell motility and adhesion, providing orthogonal information that can be used either individually or collectively in many different experimental setups. These optimized protocols will facilitate physiological and cellular characterization of these processes, which may be used for fast screening of specific therapeutic cancer drugs for migratory function, novel strategies in cancer diagnosis, and for assaying new molecules involved in adhesion and invasion metastatic properties of cancer cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Cell Adhesion and Migration, a section of the journal Frontiers in Cell and Developmental Biology Co-senior authors Edited by: Tadahide Furuno, Aichi Gakuin University, Japan Oscar Maiques, Barts Cancer Institute, Queen Mary University of London, John Vane Science Building, London, United Kingdom Present Address: Jordi Pijuan, Laboratory of Neurogenetics and Molecular Medicine, Institut de Recerca Sant Joan de Déu, Barcelona, Spain Reviewed by: Melanie D. White, Agency for Science, Technology and Research (A*STAR), Singapore; Nagaraj Balasubramanian, Indian Institute of Science Education and Research, Pune, India |
ISSN: | 2296-634X 2296-634X |
DOI: | 10.3389/fcell.2019.00107 |