A Perspective on Extracellular Vesicles Proteomics

Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to ide...

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Published inFrontiers in chemistry Vol. 5; p. 102
Main Authors Rosa-Fernandes, Livia, Rocha, Victória Bombarda, Carregari, Victor Corasolla, Urbani, Andrea, Palmisano, Giuseppe
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 21.11.2017
Subjects
bottom-up
Chemistry
exosomes
extracellular vesicles
mass spectrometry
post-translational modification
proteomics
extracellular vesicles
proteomics
bottom-up
exosomes
mass spectrometry
post-translational modification
crosslinking
top-down
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Abstract Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
AbstractList Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
Author Rosa-Fernandes, Livia
Palmisano, Giuseppe
Urbani, Andrea
Rocha, Victória Bombarda
Carregari, Victor Corasolla
AuthorAffiliation 1 GlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo , São Paulo , Brazil
2 Proteomic and Metabonomic Laboratory, Fondazione Santa Lucia , Rome , Italy
3 Institute of Biochemistry and Biochemical Clinic, Università Cattolica del Sacro Cuore , Rome , Italy
AuthorAffiliation_xml – name: 2 Proteomic and Metabonomic Laboratory, Fondazione Santa Lucia , Rome , Italy
– name: 1 GlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo , São Paulo , Brazil
– name: 3 Institute of Biochemistry and Biochemical Clinic, Università Cattolica del Sacro Cuore , Rome , Italy
Author_xml – sequence: 1
  givenname: Livia
  surname: Rosa-Fernandes
  fullname: Rosa-Fernandes, Livia
  organization: GlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
– sequence: 2
  givenname: Victória Bombarda
  surname: Rocha
  fullname: Rocha, Victória Bombarda
  organization: GlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
– sequence: 3
  givenname: Victor Corasolla
  surname: Carregari
  fullname: Carregari, Victor Corasolla
  organization: Proteomic and Metabonomic Laboratory, Fondazione Santa Lucia, Rome, Italy
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  givenname: Andrea
  surname: Urbani
  fullname: Urbani, Andrea
  organization: Institute of Biochemistry and Biochemical Clinic, Università Cattolica del Sacro Cuore, Rome, Italy
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  givenname: Giuseppe
  surname: Palmisano
  fullname: Palmisano, Giuseppe
  organization: Proteomic and Metabonomic Laboratory, Fondazione Santa Lucia, Rome, Italy
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Keywords extracellular vesicles
proteomics
bottom-up
exosomes
mass spectrometry
post-translational modification
crosslinking
top-down
Language English
License This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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This article was submitted to Chemical Biology, a section of the journal Frontiers in Chemistry
Edited by: Aline Maria Araujo Martins, University of Brasília, Brazil
Present Address: Livia Rosa-Fernandes, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
Reviewed by: Peter Kaiser, University of California, Irvine, United States; Gaurav Bhardwaj, University of Washington, United States
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702361/
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Snippet Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role...
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SubjectTerms bottom-up
Chemistry
exosomes
extracellular vesicles
mass spectrometry
post-translational modification
proteomics
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Title A Perspective on Extracellular Vesicles Proteomics
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