A Perspective on Extracellular Vesicles Proteomics

Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to ide...

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Published inFrontiers in chemistry Vol. 5; p. 102
Main Authors Rosa-Fernandes, Livia, Rocha, Victória Bombarda, Carregari, Victor Corasolla, Urbani, Andrea, Palmisano, Giuseppe
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 21.11.2017
Subjects
bottom-up
Chemistry
exosomes
extracellular vesicles
mass spectrometry
post-translational modification
proteomics
extracellular vesicles
proteomics
bottom-up
exosomes
mass spectrometry
post-translational modification
crosslinking
top-down
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Summary:Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
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This article was submitted to Chemical Biology, a section of the journal Frontiers in Chemistry
Edited by: Aline Maria Araujo Martins, University of Brasília, Brazil
Present Address: Livia Rosa-Fernandes, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
Reviewed by: Peter Kaiser, University of California, Irvine, United States; Gaurav Bhardwaj, University of Washington, United States
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2017.00102