Continuous production of human erythropoietin by immobilized recombinant L-929 cells

• Published in: Journal of fermentation and bioengineering Vol. 77; no. 1; pp. 52 - 56
• Main Authors: Terashima, Shuji, Kamihira, Masamichi, Ogawa, Tatsuya, Ohno, Masanao, Iijima, Shinji, Kobayashi, Takeshi
• Format: Journal Article
• Language: English
• Published: Osaka Elsevier B.V 1994; Society for Fermentation and Bioengineering
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Immobilization of organelles and whole cells; Immobilization techniques; Methods. Procedures. Technologies; Cell culture; Erythropoietin; Support; Rodentia; Immobilized cell; Continuous process; Bioreactor; Vertebrata; Mammalia; Mouse; Animal; Cellulose derivatives; Production; Recombinant protein; Fibroblast
• ISSN: 0922-338X
• DOI: 10.1016/0922-338X(94)90208-9

Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 10 7 cells/cm 3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.