Continuous production of human erythropoietin by immobilized recombinant L-929 cells
Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comp...
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Published in | Journal of fermentation and bioengineering Vol. 77; no. 1; pp. 52 - 56 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Osaka
Elsevier B.V
1994
Society for Fermentation and Bioengineering |
Subjects | |
Online Access | Get full text |
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Summary: | Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 10
7 cells/cm
3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0922-338X |
DOI: | 10.1016/0922-338X(94)90208-9 |