lectin from Musca domestica pupae stimulates B cell proliferation and enhances IL-12 production via ERK1/2-NF-κB signaling pathways

• Published in: Biotechnology letters Vol. 33; no. 8; pp. 1545 - 1550
• Main Authors: Cao, Xiaohong, Li, Yuanyuan, Hou, Lihua, Zhou, Minghui, Wang, Zhuo, Cui, Qi, Gao, Xiaohe, Wang, Chunling
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.08.2011; Springer Netherlands; Springer
• Subjects: Animals; Applied Microbiology; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; cell proliferation; Cell Proliferation - drug effects; Degradation; Extracellular Signal-Regulated MAP Kinases - metabolism; Fundamental and applied biological sciences. Psychology; Houseflies - chemistry; interleukin-12; Interleukin-12 - biosynthesis; Lectins; Lectins - pharmacology; Life Sciences; Lymphocyte Activation - drug effects; MAP Kinase Signaling System - drug effects; Microbiology; Musca domestica; NF-kappa B - metabolism; Original Research Paper; Pathways; Phosphorylation; Pupa - chemistry; pupae; Rats; Upstream; B cell; Lectin; pupa; Interleukin-12; Cell proliferation; Musca domestica pupa; Muscidae; Insecta; Musca domestica; Cytokine; Interleukin 12; Signaling pathway; Arthropoda; Production; Invertebrata; Diptera
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-011-0601-0

A D-galactose-specific lectin, MW = 40 kDa, had been purified from pupae of Musca domestica (MPL). MPL significantly promoted the proliferation of B cells and enhanced the production of IL-12 in a dose-dependent manner. MPL stimulated IκB-α degradation, NF-κB translocation and ERK1/2 phosphorylation which played an upstream role for NF-кB in MPL-induced B cells. Moreover, MPL regulated cell proliferation and induced IL-12 production through ERK1/2-NF-κB signaling pathway.