Evolutionary Diversity of Prophage DNA in Klebsiella pneumoniae Chromosomes

• Published in: Frontiers in microbiology Vol. 10; p. 2840
• Main Authors: Wang, Fengling, Wang, Dongguo, Hou, Wei, Jin, Qian, Feng, Jiao, Zhou, Dongsheng
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 06.12.2019
• Subjects: DNA conjugative transfer; evolutionary diversity of prophage DNA; K. pneumoniae chromosome; massive genetic acquisition or loss; Microbiology; sequence annotation and comparison; K. pneumoniae chromosome; evolutionary diversity of prophage DNA; sequence annotation and comparison; massive genetic acquisition or loss; DNA conjugative transfer
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.02840

Mobile gene elements play an important role in the continuous evolution of the prophage DNA of bacteria, promoting the emergence of new gene structures. This study explored the evolution of four strains of harboring prophages, 19051, 721005, 911021, and 675920, and 16 genomes of from GenBank. The results revealed a wide range of genetic variation in the prophage DNA inserted into the sites of chromosomes. From analysis and comparison of the sequences of the 20 prophage DNAs determined from the four strains and the 16 GenBank genomes of using high-throughput sequencing and antimicrobial susceptibility tests, we identified a novel transposon, Tn . We also identified at least nine large genetic structures with massive genetic acquisitions or losses and five hotspot sites showing a tendency to undergo insertion of gene elements such as IS , IS , IS , IS , IS , Tn , MDR, and In27 related regions as variable regions; however, the only highly conserved core genes were and among the 20 prophage DNAs. These findings provide important insights into the evolutionary diversity of bacteriophage DNA contained in .