Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation

, a member of the ARGONAUTE protein family, is exclusively expressed in pre-pachytene and pachytene stages of spermatogenesis. acts in the germ cell development and the silencing of retrotransponsons to maintain the genomic integrity and stem cell character. In the present study we investigated DNA...

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Published inFrontiers in genetics Vol. 9; p. 375
Main Authors Giebler, Maria, Greither, Thomas, Behre, Hermann M
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 20.09.2018
Subjects
epigenetics
Genetics
germ cell tumors
PIWI-LIKE 2
promoter methylation
spermatogenesis
spermatogenesis
germ cell tumors
PIWI-LIKE 2
promoter methylation
epigenetics
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Summary:, a member of the ARGONAUTE protein family, is exclusively expressed in pre-pachytene and pachytene stages of spermatogenesis. acts in the germ cell development and the silencing of retrotransponsons to maintain the genomic integrity and stem cell character. In the present study we investigated DNA methylation as potential mechanism for the regulation of human expression in cell lines related to spermatozoa precursor cells. We detected a high methylation of the promoter in TCam-2 cells, while in NT2/D1 cells the promoter was hypomethylated. Concordantly, expression is higher in NT2/D1 cells than in TCam-2 cells. By demethylation of the promoter with 5'-Aza-2'-deoxycytidine, expression in TCam-2 was increased, while in NT2/D1 no alterations in expression could be detected. In conclusion, we analyzed the DNA methylation driving expression in undifferentiated germ cell tumors and demonstrated an epigenetic basis for expression in this cell type.
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Edited by: Rui Henrique, IPO Porto, Portugal
Reviewed by: Alexandra M. Lopes, Universidade do Porto, Portugal; Michael W. Y. Chan, National Chung Cheng University, Taiwan
These authors have contributed equally to this work
This article was submitted to Epigenomics and Epigenetics, a section of the journal Frontiers in Genetics
ISSN:1664-8021
1664-8021
DOI:10.3389/fgene.2018.00375